CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00557508" target="_blank" >RIV/67985904:_____/22:00557508 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/22:00557508 RIV/00216208:11310/22:10455260

Výsledek na webu

<a href="https://journals.biologists.com/jcs/article-abstract/135/6/jcs252924/274776/CDC25B-is-required-for-the-metaphase-I-metaphase?redirectedFrom=fulltext" target="_blank" >https://journals.biologists.com/jcs/article-abstract/135/6/jcs252924/274776/CDC25B-is-required-for-the-metaphase-I-metaphase?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.252924" target="_blank" >10.1242/jcs.252924</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

Popis výsledku v původním jazyce

Mammalian oocytes are arrested at meiotic prophase I. The dual-specificity phosphatase CDC25B is essential for cyclin-dependent kinase 1 (CDK1) activation that drives resumption of meiosis. CDC25B reverses the inhibitory effect of the protein kinases WEE1 and MYT1 on CDK1 activation. Cdc25b(-/-) female mice are infertile because oocytes cannot activate CDK1. To identify a role for CDC25B following resumption of meiosis, we restored CDK1 activation in Cdc25b(-/-) oocytes by inhibiting WEE1 and MYT1, or expressing EGFP-CDC25A or constitutively active EGFP-CDK1 from microinjected complementary RNAs. Forced CDK1 activation in Cdc25b(-/- )oocytes allowed resumption of meiosis, but oocytes mostly arrested at metaphase I (MI) with intact spindles. Similarly, approximately a third of Cdc25b(-/-)( )oocytes with a reduced amount of CDC25B arrested in MI. MI-arrested Cdc25b(-/-) oocytes also displayed a transient decrease in CDK1 activity similar to Cdc25b(+/-) oocytes during the MI-MII transition, whereas Cdc25b(+/+ )oocytes exhibited only a partial anaphase-promoting complex/cyclosome activation and anaphase I entry. Thus, CDC25B is necessary for the resumption of meiosis and the MI-MII transition.

Název v anglickém jazyce

CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

Popis výsledku anglicky

Mammalian oocytes are arrested at meiotic prophase I. The dual-specificity phosphatase CDC25B is essential for cyclin-dependent kinase 1 (CDK1) activation that drives resumption of meiosis. CDC25B reverses the inhibitory effect of the protein kinases WEE1 and MYT1 on CDK1 activation. Cdc25b(-/-) female mice are infertile because oocytes cannot activate CDK1. To identify a role for CDC25B following resumption of meiosis, we restored CDK1 activation in Cdc25b(-/-) oocytes by inhibiting WEE1 and MYT1, or expressing EGFP-CDC25A or constitutively active EGFP-CDK1 from microinjected complementary RNAs. Forced CDK1 activation in Cdc25b(-/- )oocytes allowed resumption of meiosis, but oocytes mostly arrested at metaphase I (MI) with intact spindles. Similarly, approximately a third of Cdc25b(-/-)( )oocytes with a reduced amount of CDC25B arrested in MI. MI-arrested Cdc25b(-/-) oocytes also displayed a transient decrease in CDK1 activity similar to Cdc25b(+/-) oocytes during the MI-MII transition, whereas Cdc25b(+/+ )oocytes exhibited only a partial anaphase-promoting complex/cyclosome activation and anaphase I entry. Thus, CDC25B is necessary for the resumption of meiosis and the MI-MII transition.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cell Science

ISSN

0021-9533

e-ISSN

1477-9137

Svazek periodika

135

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

jcs252924

Kód UT WoS článku

000779191300001

EID výsledku v databázi Scopus

2-s2.0-85127729829