Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00565231" target="_blank" >RIV/67985904:_____/22:00565231 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/22:00565231 RIV/68378041:_____/22:00565231 RIV/00064173:_____/22:43924017 RIV/00216208:11120/22:43924017 RIV/00216208:11310/22:10448505

Výsledek na webu

<a href="https://review.jove.com/t/63505/subretinal-implantation-rpe-on-carrier-minipigs-guidelines-for" target="_blank" >https://review.jove.com/t/63505/subretinal-implantation-rpe-on-carrier-minipigs-guidelines-for</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3791/63505" target="_blank" >10.3791/63505</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care

Popis výsledku v původním jazyce

Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within the retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization of the retinal anatomy and the deterioration of visual function. The substitution of damaged RPE cells (RPEs) with in vitro cultured RPE cells using a subretinal cell carrier has shown potential for re-establishing the anatomical structure of the outer retinal layers and is, therefore, being further studied. Here, we present the principles of a surgical technique that allows for the effective subretinal transplantation of a cell carrier with cultivated RPEs into minipigs. The surgeries were performed under general anesthesia and included a standard lens-sparing three-port pars plana vitrectomy (PPV), subretinal application of a balanced salt solution (BSS), a 2.7 mm retinotomy, implantation of a nanofibrous cell carrier into the subretinal space through an additional 3.0 mm sclerotomy, fluid-air exchange (FAX), silicone oil tamponade, and closure of all the sclerotomies. This surgical approach was used in 29 surgeries (18 animals) over the past 8 years with a success rate of 93.1%. Anatomic verification of the surgical placement was carried out using in vivo fundus imaging (fundus photography and optical coherence tomography). The recommended surgical steps for the subretinal implantation of RPEs on a carrier in minipig eyes can be used in future preclinical studies using large-eye animal models.

Název v anglickém jazyce

Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care

Popis výsledku anglicky

Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within the retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization of the retinal anatomy and the deterioration of visual function. The substitution of damaged RPE cells (RPEs) with in vitro cultured RPE cells using a subretinal cell carrier has shown potential for re-establishing the anatomical structure of the outer retinal layers and is, therefore, being further studied. Here, we present the principles of a surgical technique that allows for the effective subretinal transplantation of a cell carrier with cultivated RPEs into minipigs. The surgeries were performed under general anesthesia and included a standard lens-sparing three-port pars plana vitrectomy (PPV), subretinal application of a balanced salt solution (BSS), a 2.7 mm retinotomy, implantation of a nanofibrous cell carrier into the subretinal space through an additional 3.0 mm sclerotomy, fluid-air exchange (FAX), silicone oil tamponade, and closure of all the sclerotomies. This surgical approach was used in 29 surgeries (18 animals) over the past 8 years with a success rate of 93.1%. Anatomic verification of the surgical placement was carried out using in vivo fundus imaging (fundus photography and optical coherence tomography). The recommended surgical steps for the subretinal implantation of RPEs on a carrier in minipig eyes can be used in future preclinical studies using large-eye animal models.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30207 - Ophthalmology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Jove-Journal of Visualized Experiments

ISSN

1940-087X

e-ISSN

—

Svazek periodika

2022

Číslo periodika v rámci svazku

189

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

26

Strana od-do

e63505

Kód UT WoS článku

000911862800005

EID výsledku v databázi Scopus

2-s2.0-85142851944