Inhibition of caspase-11 under inflammatory conditions suppresses chondrogenic differentiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00588292" target="_blank" >RIV/67985904:_____/24:00588292 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/24:43881232

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0040816624001265?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0040816624001265?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tice.2024.102425" target="_blank" >10.1016/j.tice.2024.102425</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of caspase-11 under inflammatory conditions suppresses chondrogenic differentiation

Popis výsledku v původním jazyce

Caspase-11 is the murine homologue of human caspases-4 and5 and is involved in mediating the inflammatory response. However, its functions are often confused and misinterpreted with the more important and better described caspase-1. Therefore, this study focused exclusively on the specific roles of caspase-11, both in cartilage formation and in the inflammatory environment. The presence of caspase-11 during mouse limb development and in chondrogenic cell cultures was investigated by immunofluorescence detection. Subsequently, the function of caspase-11 was downregulated and the affected molecules investigated. The expression analysis applied for osteo/chondrogenesis associated factors and inflammatory cytokines. Simultaneously, morphological appearance of the micromass cultures was evaluated. The results revealed that caspase-11 is physiologically present during cartilage development, but its inhibition under physiological conditions has no significant effect on chondrogenic differentiation. However, in an inflammatory environment, inhibition and downregulation of caspase-11 leads to reduced differentiation of cartilage nodules. Additionally, reduced expression of several genes including Col2a1 and Sp7 and conversely increased expression of Mmp9 were observed. In the cytokine expression panel, a significant decrease was found in molecules that, along with the inflammatory function, may also be involved in cartilage differentiation. The findings bring new information about caspase-11 in chondrogenesis and show that its downregulation under inflammatory conditions reduces cartilage formation.

Název v anglickém jazyce

Inhibition of caspase-11 under inflammatory conditions suppresses chondrogenic differentiation

Popis výsledku anglicky

Caspase-11 is the murine homologue of human caspases-4 and5 and is involved in mediating the inflammatory response. However, its functions are often confused and misinterpreted with the more important and better described caspase-1. Therefore, this study focused exclusively on the specific roles of caspase-11, both in cartilage formation and in the inflammatory environment. The presence of caspase-11 during mouse limb development and in chondrogenic cell cultures was investigated by immunofluorescence detection. Subsequently, the function of caspase-11 was downregulated and the affected molecules investigated. The expression analysis applied for osteo/chondrogenesis associated factors and inflammatory cytokines. Simultaneously, morphological appearance of the micromass cultures was evaluated. The results revealed that caspase-11 is physiologically present during cartilage development, but its inhibition under physiological conditions has no significant effect on chondrogenic differentiation. However, in an inflammatory environment, inhibition and downregulation of caspase-11 leads to reduced differentiation of cartilage nodules. Additionally, reduced expression of several genes including Col2a1 and Sp7 and conversely increased expression of Mmp9 were observed. In the cytokine expression panel, a significant decrease was found in molecules that, along with the inflammatory function, may also be involved in cartilage differentiation. The findings bring new information about caspase-11 in chondrogenesis and show that its downregulation under inflammatory conditions reduces cartilage formation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Tissue & Cell

ISSN

0040-8166

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

Aug 24

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

102425

Kód UT WoS článku

001255541800001

EID výsledku v databázi Scopus

2-s2.0-85195873853