Mitochondria as indispensable yet replaceable components of germ plasm: insights into PGCs specification in sturgeons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00643129" target="_blank" >RIV/67985904:_____/25:00643129 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/25:00643129 RIV/60076658:12520/25:43909683

Výsledek na webu

<a href="https://rep.bioscientifica.com/view/journals/rep/169/4/REP-24-0441.xml" target="_blank" >https://rep.bioscientifica.com/view/journals/rep/169/4/REP-24-0441.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-24-0441" target="_blank" >10.1530/REP-24-0441</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mitochondria as indispensable yet replaceable components of germ plasm: insights into PGCs specification in sturgeons

Popis výsledku v původním jazyce

In brief The mitochondria within germ plasm contribute to the formation and specification of primordial germ cells (PGCs) in non-teleost fishes regardless of their origin from germ plasm. This study offers new insights into germ cell biology and potential strategies for conserving matrilineal genetics in sturgeons.Abstract While it is widely recognised that mitochondria are components of germ plasm, their specific role in the formation and specification of PGCs remains poorly understood. Furthermore, it has not been established whether mitochondria in germ plasm possess unique characteristics essential for their function. In this study, we demonstrate that mitochondria are indispensable for PGC development in non-teleost fishes and that their role is not dependent on their origin from germ plasm. Using sturgeon embryos, we showed that UV radiation applied to the vegetal pole effectively eliminates germ plasm, including mitochondria, and prevents PGC formation. Remarkably, we restored germ plasm function and PGC development by injecting mitochondria derived from donor eggs, even when these mitochondria were not originally part of the germ plasm. Transplanted mitochondria were successfully identified in larval PGCs using a fluorescent PKH26 tracer, and in interspecies transplantation experiments, their presence was confirmed using species-specific mtDNA and mtRNA primers in larvae and individual PGCs. Our findings reveal that mitochondria are critical but not germ plasm-specific determinants of PGC formation. This study provides novel insights into the developmental pathways of germ cells and establishes a previously unrecognised flexibility in mitochondrial functionality within the germ line. These findings also offer a potential method for conserving matrilineal genetics in critically endangered species such as sturgeons while simultaneously opening new avenues for studying germ lines with high interspecies mitochondrial heteroplasmy and contributing to broader evolutionary and conservation biology.

Název v anglickém jazyce

Mitochondria as indispensable yet replaceable components of germ plasm: insights into PGCs specification in sturgeons

Popis výsledku anglicky

In brief The mitochondria within germ plasm contribute to the formation and specification of primordial germ cells (PGCs) in non-teleost fishes regardless of their origin from germ plasm. This study offers new insights into germ cell biology and potential strategies for conserving matrilineal genetics in sturgeons.Abstract While it is widely recognised that mitochondria are components of germ plasm, their specific role in the formation and specification of PGCs remains poorly understood. Furthermore, it has not been established whether mitochondria in germ plasm possess unique characteristics essential for their function. In this study, we demonstrate that mitochondria are indispensable for PGC development in non-teleost fishes and that their role is not dependent on their origin from germ plasm. Using sturgeon embryos, we showed that UV radiation applied to the vegetal pole effectively eliminates germ plasm, including mitochondria, and prevents PGC formation. Remarkably, we restored germ plasm function and PGC development by injecting mitochondria derived from donor eggs, even when these mitochondria were not originally part of the germ plasm. Transplanted mitochondria were successfully identified in larval PGCs using a fluorescent PKH26 tracer, and in interspecies transplantation experiments, their presence was confirmed using species-specific mtDNA and mtRNA primers in larvae and individual PGCs. Our findings reveal that mitochondria are critical but not germ plasm-specific determinants of PGC formation. This study provides novel insights into the developmental pathways of germ cells and establishes a previously unrecognised flexibility in mitochondrial functionality within the germ line. These findings also offer a potential method for conserving matrilineal genetics in critically endangered species such as sturgeons while simultaneously opening new avenues for studying germ lines with high interspecies mitochondrial heteroplasmy and contributing to broader evolutionary and conservation biology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction

ISSN

1470-1626

e-ISSN

1741-7899

Svazek periodika

169

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

e240441

Kód UT WoS článku

001518870400014

EID výsledku v databázi Scopus

2-s2.0-105001219425