Intraradical Dynamics of Two Coexisting Isolates of the Arbuscular Mycorrhizal Fungus Glomus intraradices Sensu Lato as Estimated by Real-Time PCR of Mitochondrial DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F12%3A00381085" target="_blank" >RIV/67985939:_____/12:00381085 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/12:00381085

Výsledek na webu

<a href="http://dx.doi.org/10.1128/AEM.00035-12" target="_blank" >http://dx.doi.org/10.1128/AEM.00035-12</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.00035-12" target="_blank" >10.1128/AEM.00035-12</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intraradical Dynamics of Two Coexisting Isolates of the Arbuscular Mycorrhizal Fungus Glomus intraradices Sensu Lato as Estimated by Real-Time PCR of Mitochondrial DNA

Popis výsledku v původním jazyce

We applied real-time PCR assays targeting the large subunit of mtDNA to monitor the DNA dynamics of two isolates of Glomus intraradices sensu lato coexisting in the roots of medic (Medicago sativa). The mtDNA-based quantification was compared to quantification in nrDNA. The ratio of copy numbers determined by the nrDNA- and mtDNA-based assays consistently differed between the two isolates. Within an isolate, copy numbers of the nuclear and the mitochondrial genes were closely correlated. The two quantification approaches revealed similar trends in the dynamics of both isolates, depending on whether they were inoculated alone or together. Taken together, the results of this study show that real-time PCR assays targeted to the large subunit of mtDNA maybecome useful tools for the study of coexisting AM fungi.

Název v anglickém jazyce

Intraradical Dynamics of Two Coexisting Isolates of the Arbuscular Mycorrhizal Fungus Glomus intraradices Sensu Lato as Estimated by Real-Time PCR of Mitochondrial DNA

Popis výsledku anglicky

We applied real-time PCR assays targeting the large subunit of mtDNA to monitor the DNA dynamics of two isolates of Glomus intraradices sensu lato coexisting in the roots of medic (Medicago sativa). The mtDNA-based quantification was compared to quantification in nrDNA. The ratio of copy numbers determined by the nrDNA- and mtDNA-based assays consistently differed between the two isolates. Within an isolate, copy numbers of the nuclear and the mitochondrial genes were closely correlated. The two quantification approaches revealed similar trends in the dynamics of both isolates, depending on whether they were inoculated alone or together. Taken together, the results of this study show that real-time PCR assays targeted to the large subunit of mtDNA maybecome useful tools for the study of coexisting AM fungi.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F09%2F0838" target="_blank" >GA526/09/0838: Koexistence nativních a inokulovaných arbuskulárně mykorhizních hub v kořenech hostitelských rostlin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

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Svazek periodika

78

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

3630-3637

Kód UT WoS článku

000303553900014

EID výsledku v databázi Scopus

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