A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F13%3A00393243" target="_blank" >RIV/67985939:_____/13:00393243 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0055283" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0055283</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0055283" target="_blank" >10.1371/journal.pone.0055283</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

Popis výsledku v původním jazyce

Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead.

Název v anglickém jazyce

A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

Popis výsledku anglicky

Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000315184200014

EID výsledku v databázi Scopus

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