Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F14%3A00430204" target="_blank" >RIV/67985939:_____/14:00430204 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/14:00430204 RIV/28778758:_____/14:#0000064 RIV/62156489:43210/14:00218609

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jphotobiol.2014.04.014" target="_blank" >http://dx.doi.org/10.1016/j.jphotobiol.2014.04.014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotobiol.2014.04.014" target="_blank" >10.1016/j.jphotobiol.2014.04.014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods

Popis výsledku v původním jazyce

We analyzed antibacterial effects of several novel phthalocyanines against E. coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 hoursof exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg L-1 and four of them were even efficient in the concentration of 0.20 mg L-1. Antibacterial activity of neutral andanionic compounds was considerably lower or even negligible. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines affected cytometric measurements of total cell counts, therefore this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. On the other hand, cytometric measurement of cell membrane integrity appears to be a suitable and sensitive parameter.

Název v anglickém jazyce

Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods

Popis výsledku anglicky

We analyzed antibacterial effects of several novel phthalocyanines against E. coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 hoursof exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg L-1 and four of them were even efficient in the concentration of 0.20 mg L-1. Antibacterial activity of neutral andanionic compounds was considerably lower or even negligible. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines affected cytometric measurements of total cell counts, therefore this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. On the other hand, cytometric measurement of cell membrane integrity appears to be a suitable and sensitive parameter.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Photochemistry and Photobiology. B - Biology Section

ISSN

1011-1344

e-ISSN

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Svazek periodika

138

Číslo periodika v rámci svazku

Sep 2014

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

230-239

Kód UT WoS článku

000340987500027

EID výsledku v databázi Scopus

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