DNA extraction from herbarium specimens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F14%3A00436267" target="_blank" >RIV/67985939:_____/14:00436267 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-62703-767-9_4" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-767-9_4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-767-9_4" target="_blank" >10.1007/978-1-62703-767-9_4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA extraction from herbarium specimens

Popis výsledku v původním jazyce

With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatmentof the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described.

Název v anglickém jazyce

DNA extraction from herbarium specimens

Popis výsledku anglicky

With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatmentof the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described.

Druh

C - Kapitola v odborné knize

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Molecular Plant Taxonomy: Methods and protocols

ISBN

978-1-62703-766-2

Počet stran výsledku

18

Strana od-do

67-84

Počet stran knihy

402

Název nakladatele

Springer Science+Business Media

Místo vydání

New York

Kód UT WoS kapitoly

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