The effect of different DNA isolation protocols and AFLP fingerprinting optimizations on error rate estimates in the bryophyte Campylopus introflexus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10131722" target="_blank" >RIV/00216208:11310/12:10131722 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/12:00059386

Výsledek na webu

<a href="http://www.oikos.ekol.lu.se/lindbergiapdfs/Lindbergia35.7-17.pdf" target="_blank" >http://www.oikos.ekol.lu.se/lindbergiapdfs/Lindbergia35.7-17.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of different DNA isolation protocols and AFLP fingerprinting optimizations on error rate estimates in the bryophyte Campylopus introflexus

Popis výsledku v původním jazyce

Amplified fragment length polymorphism (AFLP) has become a standard method for investigating genetic variation in plants. Nevertheless, only a few applications in bryophytes have been published and there is still a need to optimize the method. We optimized DNA isolation and AFLP protocols for Campylopus introflexus (Hedw.) Brid. As DNA quality is crucial for successful AFLP analysis, three different DNA extraction protocols were compared and the Invisorb Plant Mini Kit produced the highest DNA amount (15, 38 and 47 ng ?l-1 for dry, fresh and new cultivated tissues, respectively) and purity. Newly grown stems gave the purest DNA (absorbance at the wavelengths of 260 nm and 280 nm was 1.86). However, banding patterns obtained from dry herbarium specimens(up to two years old) corresponded with those from fresh material at a similarity level of 94.71%. The replicability of AFLP profiles was not dependent on the way plants were stored. We compared commercial kits and the influence of modif

Název v anglickém jazyce

The effect of different DNA isolation protocols and AFLP fingerprinting optimizations on error rate estimates in the bryophyte Campylopus introflexus

Popis výsledku anglicky

Amplified fragment length polymorphism (AFLP) has become a standard method for investigating genetic variation in plants. Nevertheless, only a few applications in bryophytes have been published and there is still a need to optimize the method. We optimized DNA isolation and AFLP protocols for Campylopus introflexus (Hedw.) Brid. As DNA quality is crucial for successful AFLP analysis, three different DNA extraction protocols were compared and the Invisorb Plant Mini Kit produced the highest DNA amount (15, 38 and 47 ng ?l-1 for dry, fresh and new cultivated tissues, respectively) and purity. Newly grown stems gave the purest DNA (absorbance at the wavelengths of 260 nm and 280 nm was 1.86). However, banding patterns obtained from dry herbarium specimens(up to two years old) corresponded with those from fresh material at a similarity level of 94.71%. The replicability of AFLP profiles was not dependent on the way plants were stored. We compared commercial kits and the influence of modif

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lindbergia

ISSN

0105-0761

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

22.2.

Stát vydavatele periodika

SE - Švédské království

Počet stran výsledku

11

Strana od-do

7-17

Kód UT WoS článku

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EID výsledku v databázi Scopus

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