DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F17%3AN0000002" target="_blank" >RIV/00007064:K01__/17:N0000002 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >http://dx.doi.org/10.1080/24701394.2017.1298102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >10.1080/24701394.2017.1298102</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Popis výsledku v původním jazyce

Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.

Název v anglickém jazyce

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Popis výsledku anglicky

Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/VI20162020015" target="_blank" >VI20162020015: Zavedení nových metod identifikace lidského, zvířecího a rostlinného materiálu do forenzní praxe při dokazování trestných činů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mitochondrial DNA Part A

ISSN

2470-1408

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

21 Mar 2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

1-4

Kód UT WoS článku

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EID výsledku v databázi Scopus

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