DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F18%3AN0000001" target="_blank" >RIV/00007064:K01__/18:N0000001 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60077344:_____/18:00473646 RIV/60460709:41210/18:77420
Výsledek na webu
<a href="https://www.tandfonline.com/doi/abs/10.1080/24701394.2017.1298102?journalCode=imdn21" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/24701394.2017.1298102?journalCode=imdn21</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/https://doi.org/10.1080/24701394.2017.1298102" target="_blank" >https://doi.org/10.1080/24701394.2017.1298102</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic
Popis výsledku v původním jazyce
Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.
Název v anglickém jazyce
DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic
Popis výsledku anglicky
Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10603 - Genetics and heredity (medical genetics to be 3)
Návaznosti výsledku
Projekt
<a href="/cs/project/VI20162020015" target="_blank" >VI20162020015: Zavedení nových metod identifikace lidského, zvířecího a rostlinného materiálu do forenzní praxe při dokazování trestných činů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
ISSN
2470-1394
e-ISSN
2470-1408
Svazek periodika
29
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
4
Strana od-do
427-430
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85015866868