DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77420" target="_blank" >RIV/60460709:41210/18:77420 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00007064:K01__/18:N0000001 RIV/60077344:_____/18:00473646

Výsledek na webu

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >http://dx.doi.org/10.1080/24701394.2017.1298102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >10.1080/24701394.2017.1298102</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Popis výsledku v původním jazyce

Several methods of DNA extraction, coupled with DNA barcoding species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages - eggs, the first instar larvae, and empty pupal cases (puparia) - using four different extraction methods, namely, one simple homemade extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The simple extraction buffer method was successful for half of the eggs and for the first instar larval samples. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and the DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective, the extraction was successful with regard to all sample types - eggs, larvae, and pupari

Název v anglickém jazyce

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Popis výsledku anglicky

Several methods of DNA extraction, coupled with DNA barcoding species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages - eggs, the first instar larvae, and empty pupal cases (puparia) - using four different extraction methods, namely, one simple homemade extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The simple extraction buffer method was successful for half of the eggs and for the first instar larval samples. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and the DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective, the extraction was successful with regard to all sample types - eggs, larvae, and pupari

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mitochondrial DNA Part A

ISSN

2470-1394

e-ISSN

2470-1408

Svazek periodika

29

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

427-430

Kód UT WoS článku

000435078900014

EID výsledku v databázi Scopus

2-s2.0-85015866868