Development of SSR Markers by 454 Sequencing in the Endemic Species Gentianella praecox subsp. bohemica (Gentianaceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F17%3A00477571" target="_blank" >RIV/67985939:_____/17:00477571 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10368581

Výsledek na webu

<a href="http://dx.doi.org/10.3732/apps.1600114" target="_blank" >http://dx.doi.org/10.3732/apps.1600114</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3732/apps.1600114" target="_blank" >10.3732/apps.1600114</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of SSR Markers by 454 Sequencing in the Endemic Species Gentianella praecox subsp. bohemica (Gentianaceae)

Popis výsledku v původním jazyce

Gentianella praecox subsp. bohemica (Gentianaceae) is a highly protected taxon in Europe. Polymorphic microsatellite loci were developed and used to genotype individuals of Gentianella praecox subsp. bohemica (Gentianaceae) to study the genetic structure of the remaining populations. Thirty-eight primer pairs were successfully amplified. Of these, 12 polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 180 individuals of G. praecox subsp. bohemica from six populations. Allelic richness ranged between one and nine alleles per locus. We detected a high frequency of polyploid individuals (77.8%). The highest average percentage of heterozygous genotypes was identified for samples from the Hroby population (75.5%). All loci can also be amplified in the congeneric species G. praecox subsp. praecox, G. amarella subsp. amarella, and G. obtusifolia subsp. sturmiana.

Název v anglickém jazyce

Development of SSR Markers by 454 Sequencing in the Endemic Species Gentianella praecox subsp. bohemica (Gentianaceae)

Popis výsledku anglicky

Gentianella praecox subsp. bohemica (Gentianaceae) is a highly protected taxon in Europe. Polymorphic microsatellite loci were developed and used to genotype individuals of Gentianella praecox subsp. bohemica (Gentianaceae) to study the genetic structure of the remaining populations. Thirty-eight primer pairs were successfully amplified. Of these, 12 polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 180 individuals of G. praecox subsp. bohemica from six populations. Allelic richness ranged between one and nine alleles per locus. We detected a high frequency of polyploid individuals (77.8%). The highest average percentage of heterozygous genotypes was identified for samples from the Hroby population (75.5%). All loci can also be amplified in the congeneric species G. praecox subsp. praecox, G. amarella subsp. amarella, and G. obtusifolia subsp. sturmiana.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applications in Plant Sciences

ISSN

2168-0450

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

—

Kód UT WoS článku

000393800800006

EID výsledku v databázi Scopus

2-s2.0-85009063611