Development of microsatellite markers for the perennial plant Tofieldia calyculata

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00533590" target="_blank" >RIV/67985939:_____/20:00533590 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10422579

Výsledek na webu

<a href="https://doi.org/10.1002/aps3.11327" target="_blank" >https://doi.org/10.1002/aps3.11327</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/aps3.11327" target="_blank" >10.1002/aps3.11327</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of microsatellite markers for the perennial plant Tofieldia calyculata

Popis výsledku v původním jazyce

Polymorphic microsatellite markers were developed to study genetic diversity and genetic structure of populations of the locally endangered species Tofieldia calyculata (Tofieldiaceae). Nineteen polymorphic microsatellite loci were developed using DNA-enriched libraries sequenced by Ilumina technology and were then used to genotype 101 individuals from five populations from Austria, Slovakia, Poland, and the Czech Republic. Of the markers tested, 68% were polymorphic in four of the five investigated populations, with one marker polymorphic in all populations. The number of alleles per locus in the populations ranged from one to 11. Levels of observed and expected heterozygosity ranged from 0.00 to 0.75 and from 0.00 to 0.84, respectively. Moreover, six of the 19 loci amplified when tested in the congeneric species T. pusilla. The 19 newly developed microsatellite markers can be used to describe genetic diversity and population structure of populations of T. calyculata.

Název v anglickém jazyce

Development of microsatellite markers for the perennial plant Tofieldia calyculata

Popis výsledku anglicky

Polymorphic microsatellite markers were developed to study genetic diversity and genetic structure of populations of the locally endangered species Tofieldia calyculata (Tofieldiaceae). Nineteen polymorphic microsatellite loci were developed using DNA-enriched libraries sequenced by Ilumina technology and were then used to genotype 101 individuals from five populations from Austria, Slovakia, Poland, and the Czech Republic. Of the markers tested, 68% were polymorphic in four of the five investigated populations, with one marker polymorphic in all populations. The number of alleles per locus in the populations ranged from one to 11. Levels of observed and expected heterozygosity ranged from 0.00 to 0.75 and from 0.00 to 0.84, respectively. Moreover, six of the 19 loci amplified when tested in the congeneric species T. pusilla. The 19 newly developed microsatellite markers can be used to describe genetic diversity and population structure of populations of T. calyculata.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applications in Plant Sciences

ISSN

2168-0450

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

000520981900001

EID výsledku v databázi Scopus

2-s2.0-85081742830