Genome sizes and genomic guanine+cytosine (GC) contents of the Czech vascular flora with new estimates for 1700 species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F19%3A00509593" target="_blank" >RIV/67985939:_____/19:00509593 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00107441

Výsledek na webu

<a href="http://hdl.handle.net/11104/0301481" target="_blank" >http://hdl.handle.net/11104/0301481</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.23855/preslia.2019.117" target="_blank" >10.23855/preslia.2019.117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genome sizes and genomic guanine+cytosine (GC) contents of the Czech vascular flora with new estimates for 1700 species

Popis výsledku v původním jazyce

The content of DNA in the somatic, unreplicated cell nuclei (genome size) and DNA base composition (GC content) are the basic genomic parameters that can be measured by flow cytometry. Genome size, or ploidy level, can affect many plant properties and are therefore included as important features in modern biological floras and plant trait databases. However, genomic data are still largely measured mainly for taxonomic and biosystematic purposes, and despite the popularity of flow cytometry in the Czech Republic, this information is still missing for many of the vascular plants in the Czech flora. The biological significance of the GC content is less clear compared to the genome size, which, along with the greater difficulty in measuring it, results in the absence of such information for the vastmajority of species. Here, we measure these two genomic parameters for 1908 samples of 1700 species in the Czech vascular flora. Here for the first time are reported the genome sizes of more than 1000 species and GC contents of more than 1500 species, which more than doubles the amount of information on the GC content of vascular plants. Together with the published data obtained in our laboratory using the same methods and flow cytometers, this information is now available for 1910 species that occur in the Czech Republic (~83% of this country’s permanent flora, excluding apomicts). They are summarized in a table, accompanied by information about assumed chromosome number, ploidy level and an estimate of the monoploid genome size.We further provide a descriptive analysis of this dataset, list extreme values and comment on some cytogeographically interesting findings. This dataset is the largest and most comprehensive set of genomic data covering almost the entire flora of a country. It will serve as the basis of the karyological traits section of the Czech plant trait database Pladias (www.pladias.cz) and for testing of hypotheses about the evolution and biological relevance of these genomic parameters.

Název v anglickém jazyce

Genome sizes and genomic guanine+cytosine (GC) contents of the Czech vascular flora with new estimates for 1700 species

Popis výsledku anglicky

The content of DNA in the somatic, unreplicated cell nuclei (genome size) and DNA base composition (GC content) are the basic genomic parameters that can be measured by flow cytometry. Genome size, or ploidy level, can affect many plant properties and are therefore included as important features in modern biological floras and plant trait databases. However, genomic data are still largely measured mainly for taxonomic and biosystematic purposes, and despite the popularity of flow cytometry in the Czech Republic, this information is still missing for many of the vascular plants in the Czech flora. The biological significance of the GC content is less clear compared to the genome size, which, along with the greater difficulty in measuring it, results in the absence of such information for the vastmajority of species. Here, we measure these two genomic parameters for 1908 samples of 1700 species in the Czech vascular flora. Here for the first time are reported the genome sizes of more than 1000 species and GC contents of more than 1500 species, which more than doubles the amount of information on the GC content of vascular plants. Together with the published data obtained in our laboratory using the same methods and flow cytometers, this information is now available for 1910 species that occur in the Czech Republic (~83% of this country’s permanent flora, excluding apomicts). They are summarized in a table, accompanied by information about assumed chromosome number, ploidy level and an estimate of the monoploid genome size.We further provide a descriptive analysis of this dataset, list extreme values and comment on some cytogeographically interesting findings. This dataset is the largest and most comprehensive set of genomic data covering almost the entire flora of a country. It will serve as the basis of the karyological traits section of the Czech plant trait database Pladias (www.pladias.cz) and for testing of hypotheses about the evolution and biological relevance of these genomic parameters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Preslia

ISSN

0032-7786

e-ISSN

—

Svazek periodika

91

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

26

Strana od-do

117-142

Kód UT WoS článku

000472149600002

EID výsledku v databázi Scopus

2-s2.0-85069545715