Application-based guidelines for best practices in plant flow cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00561312" target="_blank" >RIV/67985939:_____/22:00561312 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00125097 RIV/60076658:12310/22:43904742 RIV/00216208:11310/22:10474746

Výsledek na webu

<a href="https://doi.org/10.1002/cyto.a.24499" target="_blank" >https://doi.org/10.1002/cyto.a.24499</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.a.24499" target="_blank" >10.1002/cyto.a.24499</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application-based guidelines for best practices in plant flow cytometry

Popis výsledku v původním jazyce

Flow cytometry (FCM) is currently the most widely-used method to establish nuclear DNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are sufficient for most plant applications, are commercially available at a reasonable price, the number of laboratories equipped with these instruments, and consequently new FCM users, has greatly increased over the last decade. This paper meets an urgent need for comprehensive recommendations for best practices in FCM for different plant science applications. We discuss advantages and limitations of establishing plant ploidy, genome size, DNA base composition, cell cycle activity, and level of endoreduplication. Applications of such measurements in plant systematics, ecology, molecular biology research, reproduction biology, tissue cultures, plant breeding, and seed sciences are described. Advice is included on how to obtain accurate and reliable results, as well as how to manage troubleshooting that may occur during sample preparation, cytometric measurements, and data handling. Each section is followed by best practice recommendations, tips as to what specific information should be provided in FCM papers are also provided.

Název v anglickém jazyce

Application-based guidelines for best practices in plant flow cytometry

Popis výsledku anglicky

Flow cytometry (FCM) is currently the most widely-used method to establish nuclear DNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are sufficient for most plant applications, are commercially available at a reasonable price, the number of laboratories equipped with these instruments, and consequently new FCM users, has greatly increased over the last decade. This paper meets an urgent need for comprehensive recommendations for best practices in FCM for different plant science applications. We discuss advantages and limitations of establishing plant ploidy, genome size, DNA base composition, cell cycle activity, and level of endoreduplication. Applications of such measurements in plant systematics, ecology, molecular biology research, reproduction biology, tissue cultures, plant breeding, and seed sciences are described. Advice is included on how to obtain accurate and reliable results, as well as how to manage troubleshooting that may occur during sample preparation, cytometric measurements, and data handling. Each section is followed by best practice recommendations, tips as to what specific information should be provided in FCM papers are also provided.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry. Part A

ISSN

1552-4922

e-ISSN

1552-4930

Svazek periodika

101

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

33

Strana od-do

749-781

Kód UT WoS článku

000700954500001

EID výsledku v databázi Scopus

2-s2.0-85112747267