Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10130256" target="_blank" >RIV/00216208:11310/12:10130256 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985939:_____/12:00385584 RIV/60076658:12310/12:43883647
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s10577-012-9277-0" target="_blank" >http://dx.doi.org/10.1007/s10577-012-9277-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10577-012-9277-0" target="_blank" >10.1007/s10577-012-9277-0</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples
Popis výsledku v původním jazyce
Flow cytometry (FCM) has been widely used in plant science to determine the amount of nuclear DNA, either in absolute units or in relative terms, as an indicator of ploidy. The requirement for fresh material in some applications, however, limits the value of FCM in field research, including plant biosystematics, ecology and population biology. Dried plant samples have proven to be a suitable alternative in some cases (large-scale ploidy screening) although tissue dehydration is often associated with a decrease in the quality of FCM analysis. The present study tested, using time-scale laboratory and in situ field experiments, the applicability of glycerol-treated nuclear suspension for DNA flow cytometry. We demonstrate that plant nuclei preserved in ice-cold buffer + glycerol solution remain intact for at least a few weeks and provide estimates of nuclear DNA content that are highly comparable and of similar quality to those obtained from fresh tissue. The protocol is compatible with b
Název v anglickém jazyce
Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples
Popis výsledku anglicky
Flow cytometry (FCM) has been widely used in plant science to determine the amount of nuclear DNA, either in absolute units or in relative terms, as an indicator of ploidy. The requirement for fresh material in some applications, however, limits the value of FCM in field research, including plant biosystematics, ecology and population biology. Dried plant samples have proven to be a suitable alternative in some cases (large-scale ploidy screening) although tissue dehydration is often associated with a decrease in the quality of FCM analysis. The present study tested, using time-scale laboratory and in situ field experiments, the applicability of glycerol-treated nuclear suspension for DNA flow cytometry. We demonstrate that plant nuclei preserved in ice-cold buffer + glycerol solution remain intact for at least a few weeks and provide estimates of nuclear DNA content that are highly comparable and of similar quality to those obtained from fresh tissue. The protocol is compatible with b
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chromosome Research
ISSN
0967-3849
e-ISSN
—
Svazek periodika
20
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
13
Strana od-do
303-315
Kód UT WoS článku
000302325300005
EID výsledku v databázi Scopus
—