Plant material selection, collection, preservation, and storage for nuclear DNA content estimation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00566766" target="_blank" >RIV/67985939:_____/22:00566766 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10456548 RIV/60076658:12310/22:43905646

Výsledek na webu

<a href="https://doi.org/10.1002/cyto.a.24482" target="_blank" >https://doi.org/10.1002/cyto.a.24482</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.a.24482" target="_blank" >10.1002/cyto.a.24482</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Plant material selection, collection, preservation, and storage for nuclear DNA content estimation

Popis výsledku v původním jazyce

In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear DNA content estimation using flow cytometry (FCM). While this certainly opens a wide variety of possible applications of FCM, especially when compared to classical karyological techniques restricted to tissues with active cell division, tissue selection and quality may directly affect the precision (and sometimes even reliability) of FCM measurements. It is usually convenient to first consider the goals of the study to either aim for the highest possible accuracy of estimates (e.g., for inferring genome size, detecting homoploid intraspecific genome size variation, aneuploidy, among others), or to decide that histograms of reasonable resolution provide sufficient information (e.g., ploidy level screening within a single model species). Here, a set of best practices guidelines for selecting the optimal plant tissue for FCM analysis, sampling of material, and material preservation and storage are provided. In addition, factors potentially compromising the quality of FCM estimates of nuclear DNA content and data interpretation are discussed.

Název v anglickém jazyce

Plant material selection, collection, preservation, and storage for nuclear DNA content estimation

Popis výsledku anglicky

In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear DNA content estimation using flow cytometry (FCM). While this certainly opens a wide variety of possible applications of FCM, especially when compared to classical karyological techniques restricted to tissues with active cell division, tissue selection and quality may directly affect the precision (and sometimes even reliability) of FCM measurements. It is usually convenient to first consider the goals of the study to either aim for the highest possible accuracy of estimates (e.g., for inferring genome size, detecting homoploid intraspecific genome size variation, aneuploidy, among others), or to decide that histograms of reasonable resolution provide sufficient information (e.g., ploidy level screening within a single model species). Here, a set of best practices guidelines for selecting the optimal plant tissue for FCM analysis, sampling of material, and material preservation and storage are provided. In addition, factors potentially compromising the quality of FCM estimates of nuclear DNA content and data interpretation are discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA20-22783S" target="_blank" >GA20-22783S: Genomová duplikace jako nedokonalá speciační bariéra? Evoluční mechanismy a důsledky mezi-ploidní introgrese v přírodních populacích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry. Part A

ISSN

1552-4922

e-ISSN

1552-4930

Svazek periodika

101

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

737-748

Kód UT WoS článku

000672491100001

EID výsledku v databázi Scopus

2-s2.0-85109739806