Cytometrická a mikroskopická analýza vlivu kyseliny taninové na buněčná jádra rostlin a měření obsahu DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F06%3A00081801" target="_blank" >RIV/61389030:_____/06:00081801 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content

Popis výsledku v původním jazyce

Flow cytometry (FCM) is extensively used to estimate DNA ploidy and genome size in plants. In order to determine nuclear DNA content, nuclei in suspension are stained by a DNA-specific fluorochrome and fluorescence emission is quantified. Recent studieshave shown that cytosolic compounds may interfere with binding of fluorochromes to DNA, leading to flawed data. Tannic acid, a common phenolic compound, may be responsible for some of the stoichiometric errors, especially in woody plants. In this study,the effect of tannic acid on estimation of nuclear DNA content was evaluated in Pisum sativum and Zea mays, which were chosen as model species. - Methods Nuclear suspensions were prepared from P. sativum leaf tissue using four different lysis buffers (Galbraith?s, LB01, Otto?s and Tris.MgCl2). The suspensions were treated with tannic acid (TA) at 13 different initial concentrations ranging from 0.25 to 3.50 mg mL-1. After propidium iodide (PI) staining, samples were analysed using FCM. I

Název v anglickém jazyce

Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content

Popis výsledku anglicky

Flow cytometry (FCM) is extensively used to estimate DNA ploidy and genome size in plants. In order to determine nuclear DNA content, nuclei in suspension are stained by a DNA-specific fluorochrome and fluorescence emission is quantified. Recent studieshave shown that cytosolic compounds may interfere with binding of fluorochromes to DNA, leading to flawed data. Tannic acid, a common phenolic compound, may be responsible for some of the stoichiometric errors, especially in woody plants. In this study,the effect of tannic acid on estimation of nuclear DNA content was evaluated in Pisum sativum and Zea mays, which were chosen as model species. - Methods Nuclear suspensions were prepared from P. sativum leaf tissue using four different lysis buffers (Galbraith?s, LB01, Otto?s and Tris.MgCl2). The suspensions were treated with tannic acid (TA) at 13 different initial concentrations ranging from 0.25 to 3.50 mg mL-1. After propidium iodide (PI) staining, samples were analysed using FCM. I

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06004" target="_blank" >LC06004: Integrovaný výzkum rostlinného genomu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Botany

ISSN

0305-7364

e-ISSN

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Svazek periodika

98

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

515-527

Kód UT WoS článku

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EID výsledku v databázi Scopus

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