IL-10 neovlivňuje oxidativní vzplanutí a expresi vybraných povrchových antigenů na lidských krevních fagocytech in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F04%3A00104649" target="_blank" >RIV/68081707:_____/04:00104649 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

IL-10 does not affect oxidative burst and expression of selected surface antigen on human blood phagocytes in vitro

Popis výsledku v původním jazyce

Cytokines play a major role in the control of inflammatory responses, participate in the regulation of blood phagocyte activities and as such are used for immunomodulatory therapy. In the present Study, the influence of IL-10 on human blood phagocyte activity in the presence/absence of IL-6, IL-8 and TNF-alpha was tested in vitro. Our research analyzed the effects of cytokines on the production of reactive oxygen species measured by chemiluminescence and flow cytometry. and on the expression of surfacemolecules (CD11b, CD15, CD62L, CD31) measured by flow cytometry. IL-10 had no inhibitory effect on reactive oxygen species production and the expression of any examined adhesion molecule by resting or stimulated blood phagocytes within 3 h of incubation.Conversely, TNF-alpha, IL-6, and IL-8 increased reactive oxygen species production and the expression of CDllb and CD15 on both neutrophils and monocytes and decreased the expression of CD62L.

Název v anglickém jazyce

IL-10 does not affect oxidative burst and expression of selected surface antigen on human blood phagocytes in vitro

Popis výsledku anglicky

Cytokines play a major role in the control of inflammatory responses, participate in the regulation of blood phagocyte activities and as such are used for immunomodulatory therapy. In the present Study, the influence of IL-10 on human blood phagocyte activity in the presence/absence of IL-6, IL-8 and TNF-alpha was tested in vitro. Our research analyzed the effects of cytokines on the production of reactive oxygen species measured by chemiluminescence and flow cytometry. and on the expression of surfacemolecules (CD11b, CD15, CD62L, CD31) measured by flow cytometry. IL-10 had no inhibitory effect on reactive oxygen species production and the expression of any examined adhesion molecule by resting or stimulated blood phagocytes within 3 h of incubation.Conversely, TNF-alpha, IL-6, and IL-8 increased reactive oxygen species production and the expression of CDllb and CD15 on both neutrophils and monocytes and decreased the expression of CD62L.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/IBS5004009" target="_blank" >IBS5004009: Netradiční terapeutické přístupy v onkologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

199-208

Kód UT WoS článku

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EID výsledku v databázi Scopus

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