Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00428666" target="_blank" >RIV/68081707:_____/14:00428666 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.lfs.2014.01.082" target="_blank" >http://dx.doi.org/10.1016/j.lfs.2014.01.082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.lfs.2014.01.082" target="_blank" >10.1016/j.lfs.2014.01.082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

Popis výsledku v původním jazyce

Aims: The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. Main methods: Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol-HRP-H2O2 based CL). Key findings: Histamine, 4-methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP-activated whole blood CL in a dose-dependent manner.

Název v anglickém jazyce

Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

Popis výsledku anglicky

Aims: The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. Main methods: Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol-HRP-H2O2 based CL). Key findings: Histamine, 4-methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP-activated whole blood CL in a dose-dependent manner.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/LD11010" target="_blank" >LD11010: Vliv antagonistů histaminového receptoru H4R na tvorbu reaktivních metabolitů kyslíku a dusíku fagocyty</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Life Sciences

ISSN

0024-3205

e-ISSN

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Svazek periodika

100

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

67-72

Kód UT WoS článku

000334323600009

EID výsledku v databázi Scopus

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