Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00506827" target="_blank" >RIV/68081707:_____/17:00506827 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.researchgate.net/publication/314265979_Chemiluminescence_A_Sensitive_Method_for_Detecting_the_Effects_of_Histamine_Receptor_AgonistsAntagonists_on_Neutrophil_Oxidative_Burst" target="_blank" >https://www.researchgate.net/publication/314265979_Chemiluminescence_A_Sensitive_Method_for_Detecting_the_Effects_of_Histamine_Receptor_AgonistsAntagonists_on_Neutrophil_Oxidative_Burst</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-6843-5_7" target="_blank" >10.1007/978-1-4939-6843-5_7</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst
Popis výsledku v původním jazyce
Polymorphonuclear leukocytes (neutrophils) form the first line of defence in humans. After activation, they migrate through vessel walls toward the invading agent. The intruding pathogens are eliminated through the action of released proteases and by production of reactive oxygen species (ROS). Histamine, a biogenic amine with various physiological and pathological effects, is known to modulate the immune response of neutrophils, e.g., it blunts the production of ROS in neutrophils after binding to the histamine-2 receptor. To detect the ROS in neutrophils, various methods are used. Among these, luminophore-enhanced chemiluminescence is a very sensitive and easy tool for studying the effects of various compounds on ROS production in neutrophils. On the other hand, its selectivity is questionable. However, employing cell-free systems producing selective ROS types, the inhibitory effects of tested compounds against distinct ROS may be evaluated. In this chapter, we describe the methods for the evaluation of ROS modulating effects of histamine agonists/antagonists in human neutrophils by using chemiluminescence methods.
Název v anglickém jazyce
Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst
Popis výsledku anglicky
Polymorphonuclear leukocytes (neutrophils) form the first line of defence in humans. After activation, they migrate through vessel walls toward the invading agent. The intruding pathogens are eliminated through the action of released proteases and by production of reactive oxygen species (ROS). Histamine, a biogenic amine with various physiological and pathological effects, is known to modulate the immune response of neutrophils, e.g., it blunts the production of ROS in neutrophils after binding to the histamine-2 receptor. To detect the ROS in neutrophils, various methods are used. Among these, luminophore-enhanced chemiluminescence is a very sensitive and easy tool for studying the effects of various compounds on ROS production in neutrophils. On the other hand, its selectivity is questionable. However, employing cell-free systems producing selective ROS types, the inhibitory effects of tested compounds against distinct ROS may be evaluated. In this chapter, we describe the methods for the evaluation of ROS modulating effects of histamine agonists/antagonists in human neutrophils by using chemiluminescence methods.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Histamine Receptors as Drug Targets
ISSN
1557-2153
e-ISSN
—
Svazek periodika
—
Číslo periodika v rámci svazku
2017
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
26
Strana od-do
183-208
Kód UT WoS článku
000412310500008
EID výsledku v databázi Scopus
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