The histamine H-4 receptor is a potent inhibitor of adhesion-dependent degranulation in human neutrophils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00431753" target="_blank" >RIV/68081707:_____/14:00431753 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1189/jlb.2AB0813-432RR" target="_blank" >http://dx.doi.org/10.1189/jlb.2AB0813-432RR</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1189/jlb.2AB0813-432RR" target="_blank" >10.1189/jlb.2AB0813-432RR</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The histamine H-4 receptor is a potent inhibitor of adhesion-dependent degranulation in human neutrophils

Popis výsledku v původním jazyce

The histamine H-4 receptor regulates the inflammatory response. However, it is not known whether this receptor has a functional role in human neutrophils. We found that fMLP (1 mu M), but not histamine (0.1-1 mu M), induced Mac-1-dependent adhesion, polarization, and degranulation (release of lactoferrin). A pretreatment of neutrophils with histamine (0.001-1 mu M) or JNJ 28610244 (0.1-10 mu M), a specific H-4 receptor agonist, led to inhibition of degranulation. Total inhibition of degranulation was obtained with 0.1 mu M histamine and 10 mu M JNJ 28610244. Furthermore, such inhibition by histamine of degranulation was reversed by JNJ 7777120 and JNJ 28307474, two selective H-4 receptor antagonists. However, neither histamine nor the H-4 receptor agonist JNJ 28610244 prevented fMLP-induced, Mac-1-dependent adhesion, indicating that the H-4 receptor may block signals emanating from Mac-1-controlling degranulation. Likewise, engagement of the H-4 receptor by the selective agonist JNJ 28

Název v anglickém jazyce

The histamine H-4 receptor is a potent inhibitor of adhesion-dependent degranulation in human neutrophils

Popis výsledku anglicky

The histamine H-4 receptor regulates the inflammatory response. However, it is not known whether this receptor has a functional role in human neutrophils. We found that fMLP (1 mu M), but not histamine (0.1-1 mu M), induced Mac-1-dependent adhesion, polarization, and degranulation (release of lactoferrin). A pretreatment of neutrophils with histamine (0.001-1 mu M) or JNJ 28610244 (0.1-10 mu M), a specific H-4 receptor agonist, led to inhibition of degranulation. Total inhibition of degranulation was obtained with 0.1 mu M histamine and 10 mu M JNJ 28610244. Furthermore, such inhibition by histamine of degranulation was reversed by JNJ 7777120 and JNJ 28307474, two selective H-4 receptor antagonists. However, neither histamine nor the H-4 receptor agonist JNJ 28610244 prevented fMLP-induced, Mac-1-dependent adhesion, indicating that the H-4 receptor may block signals emanating from Mac-1-controlling degranulation. Likewise, engagement of the H-4 receptor by the selective agonist JNJ 28

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0030" target="_blank" >EE2.3.30.0030: Rozvoj lidských zdrojů pro oblast buněčné biologie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Leukocyte Biology

ISSN

0741-5400

e-ISSN

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Svazek periodika

96

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

411-418

Kód UT WoS článku

000340829400007

EID výsledku v databázi Scopus

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