Direct chemical modification and voltammetric detection of glycans in glycoproteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00440098" target="_blank" >RIV/68081707:_____/14:00440098 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00209805:_____/14:#0000582

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.elecom.2014.08.011" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2014.08.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.elecom.2014.08.011" target="_blank" >10.1016/j.elecom.2014.08.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct chemical modification and voltammetric detection of glycans in glycoproteins

Popis výsledku v původním jazyce

Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Direct chemical modification and voltammetric detection of glycans in glycoproteins

Popis výsledku anglicky

Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochemistry Communications

ISSN

1388-2481

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

NOV2014

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

52-55

Kód UT WoS článku

000345208000014

EID výsledku v databázi Scopus

—