Shape-selective recognition of DNA abasic sites by metallohelices: inhibition of human AP endonuclease 1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00457638" target="_blank" >RIV/68081707:_____/15:00457638 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gkv438" target="_blank" >http://dx.doi.org/10.1093/nar/gkv438</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkv438" target="_blank" >10.1093/nar/gkv438</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Shape-selective recognition of DNA abasic sites by metallohelices: inhibition of human AP endonuclease 1

Popis výsledku v původním jazyce

Loss of a base in DNA leading to creation of an abasic (AP) site leaving a deoxyribose residue in the strand, is a frequent lesion that may occur spontaneously or under the action of various physical and chemical agents. Progress in the understanding ofthe chemistry and enzymology of abasic DNA largely relies upon the study of AP sites in synthetic duplexes. We report here on interactions of diastereomerically pure metallo-helical 'flexicate' complexes, bimetallic triple-stranded ferro-helicates [Fe-2(NN-NN)(3)](4+) incorporating the common NN-NN bis(bidentate) helicand, with short DNA duplexes containing AP sites in different sequence contexts. The results show that the flexicates bind to AP sites in DNA duplexes in a shape-selective manner. They preferentially bind to AP sites flanked by purines on both sides and their binding is enhanced when a pyrimidine is placed in opposite orientation to the lesion. Notably, the Lambda-enantiomer binds to all tested AP sites with higher affinit

Název v anglickém jazyce

Shape-selective recognition of DNA abasic sites by metallohelices: inhibition of human AP endonuclease 1

Popis výsledku anglicky

Loss of a base in DNA leading to creation of an abasic (AP) site leaving a deoxyribose residue in the strand, is a frequent lesion that may occur spontaneously or under the action of various physical and chemical agents. Progress in the understanding ofthe chemistry and enzymology of abasic DNA largely relies upon the study of AP sites in synthetic duplexes. We report here on interactions of diastereomerically pure metallo-helical 'flexicate' complexes, bimetallic triple-stranded ferro-helicates [Fe-2(NN-NN)(3)](4+) incorporating the common NN-NN bis(bidentate) helicand, with short DNA duplexes containing AP sites in different sequence contexts. The results show that the flexicates bind to AP sites in DNA duplexes in a shape-selective manner. They preferentially bind to AP sites flanked by purines on both sides and their binding is enhanced when a pyrimidine is placed in opposite orientation to the lesion. Notably, the Lambda-enantiomer binds to all tested AP sites with higher affinit

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP205%2F11%2F0856" target="_blank" >GAP205/11/0856: Termodynamická analýza DNA poškozené fyzikálními a chemickými agens</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

1362-4962

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

5297-5306

Kód UT WoS článku

000357886900011

EID výsledku v databázi Scopus

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