Photo-induced DNA cleavage and cytotoxicity of a ruthenium(II) arene anticancer complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00461985" target="_blank" >RIV/68081707:_____/16:00461985 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/16:33161146

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jinorgbio.2015.12.029" target="_blank" >http://dx.doi.org/10.1016/j.jinorgbio.2015.12.029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jinorgbio.2015.12.029" target="_blank" >10.1016/j.jinorgbio.2015.12.029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Photo-induced DNA cleavage and cytotoxicity of a ruthenium(II) arene anticancer complex

Popis výsledku v původním jazyce

We report DNA cleavage by ruthenium(II) arene anticancer complex [(eta(6)-p-terp)Ru-II(en)Cl](+) (p-terp = paraterphenyl, en = 1,2-diaminoethane, complex 1) after its photoactivation by UVA and visible light, and the toxic effects of photoactivated 1 in cancer cells. It was shown in our previous work (T. Bugarcic et al., J. Med. Chem. 51 (2008) 5310-5319) that this complex exhibits promising toxic effects in several human tumor cell lines and concomitantly its DNA binding mode involves combined intercalative and monofunctional (coordination) binding modes. We demonstrate in the present work that when photoactivated by UVA or visible light, 1 efficiently photocleaves DNA, also in hypoxic media. Studies of the mechanism underlying DNA cleavage by photoactivated 1 reveal that the photocleavage reaction does not involve generation of reactive oxygen species (ROS), although contribution of singlet oxygen (O-1(2)) to the DNA photocleavage process cannot be entirely excluded. Notably, the mechanism of DNA photocleavage by 1 appears to involve a direct modification of mainly those guanine residues to which 1 is coordinatively bound.

Název v anglickém jazyce

Photo-induced DNA cleavage and cytotoxicity of a ruthenium(II) arene anticancer complex

Popis výsledku anglicky

We report DNA cleavage by ruthenium(II) arene anticancer complex [(eta(6)-p-terp)Ru-II(en)Cl](+) (p-terp = paraterphenyl, en = 1,2-diaminoethane, complex 1) after its photoactivation by UVA and visible light, and the toxic effects of photoactivated 1 in cancer cells. It was shown in our previous work (T. Bugarcic et al., J. Med. Chem. 51 (2008) 5310-5319) that this complex exhibits promising toxic effects in several human tumor cell lines and concomitantly its DNA binding mode involves combined intercalative and monofunctional (coordination) binding modes. We demonstrate in the present work that when photoactivated by UVA or visible light, 1 efficiently photocleaves DNA, also in hypoxic media. Studies of the mechanism underlying DNA cleavage by photoactivated 1 reveal that the photocleavage reaction does not involve generation of reactive oxygen species (ROS), although contribution of singlet oxygen (O-1(2)) to the DNA photocleavage process cannot be entirely excluded. Notably, the mechanism of DNA photocleavage by 1 appears to involve a direct modification of mainly those guanine residues to which 1 is coordinatively bound.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Inorganic Biochemistry

ISSN

0162-0134

e-ISSN

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Svazek periodika

160

Číslo periodika v rámci svazku

JUL2016

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

149-155

Kód UT WoS článku

000378965200017

EID výsledku v databázi Scopus

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