Inhibition of nuclear factor kappaB proteins-platinated DNA interactions correlates with cytotoxic effectiveness of the platinum complexes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00470811" target="_blank" >RIV/68081707:_____/16:00470811 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/16:33161145

Výsledek na webu

<a href="http://dx.doi.org/10.1038/srep28474" target="_blank" >http://dx.doi.org/10.1038/srep28474</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep28474" target="_blank" >10.1038/srep28474</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of nuclear factor kappaB proteins-platinated DNA interactions correlates with cytotoxic effectiveness of the platinum complexes

Popis výsledku v původním jazyce

Nuclear DNA is the target responsible for anticancer activity of platinum anticancer drugs. Their activity is mediated by altered signals related to programmed cell death and the activation of various signaling pathways. An example is activation of nuclear factor kappaB (NF-kappa B). Binding of NF-kappa B proteins to their consensus sequences in DNA (kappa B sites) is the key biochemical activity responsible for the biological functions of NF-kappa B. Using gel-mobility-shift assays and surface plasmon resonance spectroscopy we examined the interactions of NF-kappa B proteins with oligodeoxyribonucleotide duplexes containing kappa B site damaged by DNA adducts of three platinum complexes. These complexes markedly differed in their toxic effects in tumor cells and comprised highly cytotoxic trinuclear platinum(II) complex BBR3464, less cytotoxic conventional cisplatin and ineffective transplatin. The results indicate that structurally different DNA adducts of these platinum complexes exhibit a different efficiency to affect the affinity of the platinated DNA (kappa B sites) to NF-kappa B proteins. Our results support the hypothesis that structural perturbations induced in DNA by platinum(II) complexes correlate with their higher efficiency to inhibit binding of NF-kappa B proteins to their kappa B sites and cytotoxicity as well. However, the full generalization of this hypothesis will require to evaluate a larger series of platinum(II) complexes.

Název v anglickém jazyce

Inhibition of nuclear factor kappaB proteins-platinated DNA interactions correlates with cytotoxic effectiveness of the platinum complexes

Popis výsledku anglicky

Nuclear DNA is the target responsible for anticancer activity of platinum anticancer drugs. Their activity is mediated by altered signals related to programmed cell death and the activation of various signaling pathways. An example is activation of nuclear factor kappaB (NF-kappa B). Binding of NF-kappa B proteins to their consensus sequences in DNA (kappa B sites) is the key biochemical activity responsible for the biological functions of NF-kappa B. Using gel-mobility-shift assays and surface plasmon resonance spectroscopy we examined the interactions of NF-kappa B proteins with oligodeoxyribonucleotide duplexes containing kappa B site damaged by DNA adducts of three platinum complexes. These complexes markedly differed in their toxic effects in tumor cells and comprised highly cytotoxic trinuclear platinum(II) complex BBR3464, less cytotoxic conventional cisplatin and ineffective transplatin. The results indicate that structurally different DNA adducts of these platinum complexes exhibit a different efficiency to affect the affinity of the platinated DNA (kappa B sites) to NF-kappa B proteins. Our results support the hypothesis that structural perturbations induced in DNA by platinum(II) complexes correlate with their higher efficiency to inhibit binding of NF-kappa B proteins to their kappa B sites and cytotoxicity as well. However, the full generalization of this hypothesis will require to evaluate a larger series of platinum(II) complexes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/LH13096" target="_blank" >LH13096: Interakce DNA modifikované platinovými cytostatiky s proteiny, které rozlišují poškozenou DNA</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

AUG2016

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000382157100001

EID výsledku v databázi Scopus

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