Structure of a Stable G-Hairpin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00476559" target="_blank" >RIV/68081707:_____/17:00476559 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/17:00094788

Výsledek na webu

<a href="http://dx.doi.org/10.1021/jacs.6b10786" target="_blank" >http://dx.doi.org/10.1021/jacs.6b10786</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/jacs.6b10786" target="_blank" >10.1021/jacs.6b10786</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure of a Stable G-Hairpin

Popis výsledku v původním jazyce

In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NIcarbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.

Název v anglickém jazyce

Structure of a Stable G-Hairpin

Popis výsledku anglicky

In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NIcarbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the American Chemical Society

ISSN

0002-7863

e-ISSN

—

Svazek periodika

139

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

3591-3594

Kód UT WoS článku

000396801300002

EID výsledku v databázi Scopus

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