Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00501578" target="_blank" >RIV/68081707:_____/18:00501578 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/slct.201803565" target="_blank" >http://dx.doi.org/10.1002/slct.201803565</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/slct.201803565" target="_blank" >10.1002/slct.201803565</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry

Popis výsledku v původním jazyce

Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site.

Název v anglickém jazyce

Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry

Popis výsledku anglicky

Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA17-09436S" target="_blank" >GA17-09436S: Translézová syntéza DNA přes léze vyvolané biologicky významnými agens z pohledu energetiky, kinetiky a mechanismu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemistrySelect

ISSN

2365-6549

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

46

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

13076-13080

Kód UT WoS článku

000453576900014

EID výsledku v databázi Scopus

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