MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00509780" target="_blank" >RIV/68081707:_____/19:00509780 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/19:00108234
Výsledek na webu
<a href="http://downloads.hindawi.com/journals/sci/2019/5128135.pdf" target="_blank" >http://downloads.hindawi.com/journals/sci/2019/5128135.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2019/5128135" target="_blank" >10.1155/2019/5128135</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
Popis výsledku v původním jazyce
The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.
Název v anglickém jazyce
MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
Popis výsledku anglicky
The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
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OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA17-05466S" target="_blank" >GA17-05466S: Role kanonické signální dráhy Wnt v neurogenezi.</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Stem Cells International
ISSN
1687-966X
e-ISSN
—
Svazek periodika
2019
Číslo periodika v rámci svazku
2019
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
17
Strana od-do
5128135
Kód UT WoS článku
000472008000001
EID výsledku v databázi Scopus
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