Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00509884" target="_blank" >RIV/68081707:_____/19:00509884 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.nature.com/articles/s41598-019-44388-9.pdf" target="_blank" >https://www.nature.com/articles/s41598-019-44388-9.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-44388-9" target="_blank" >10.1038/s41598-019-44388-9</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells
Popis výsledku v původním jazyce
AS1411 is a G-rich DNA oligonucleotide that functions as an aptamer of the protein nucleolin, found at high levels on the surface of cancer cells but not on the surface of normal cells. Herein, we have studied AS1411 as a supramolecular carrier for the delivery of an acridine-based G-quadruplex ligand, C-8, to HeLa cancer cells. Two AS1411 derivatives, LNA-AS1411 and U-AS1411, were also tested, in an attempt to compare AS1411 pharmacological properties. The results showed that AS1411-C-8 complexation was made with great binding strength and that it lowered the ligand's cytotoxicity towards non-malignant cells. This effect was suggested to be due to a decreased internalization of the complexed versus free C-8 as shown by flow cytometry. The AS1411 derivatives, despite forming a stable complex with C-8, lacked the necessary tumour-selective behaviour. The binding of C-8 to AS1411 G-quadruplex structure did not negatively affect the recognition of nucleolin by the aptamer. The AS1411-C-8 repressed c-MYC expression at the transcriptional level, possibly due to C-8 ability to stabilize the c-MYC promoter G-quadruplexes. Overall, this study demonstrates the usefulness of AS1411 as a supramolecular carrier of the G-quadruplex binder C-8 and the potential of using its tumour-selective properties for the delivery of ligands for cancer therapy.
Název v anglickém jazyce
Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells
Popis výsledku anglicky
AS1411 is a G-rich DNA oligonucleotide that functions as an aptamer of the protein nucleolin, found at high levels on the surface of cancer cells but not on the surface of normal cells. Herein, we have studied AS1411 as a supramolecular carrier for the delivery of an acridine-based G-quadruplex ligand, C-8, to HeLa cancer cells. Two AS1411 derivatives, LNA-AS1411 and U-AS1411, were also tested, in an attempt to compare AS1411 pharmacological properties. The results showed that AS1411-C-8 complexation was made with great binding strength and that it lowered the ligand's cytotoxicity towards non-malignant cells. This effect was suggested to be due to a decreased internalization of the complexed versus free C-8 as shown by flow cytometry. The AS1411 derivatives, despite forming a stable complex with C-8, lacked the necessary tumour-selective behaviour. The binding of C-8 to AS1411 G-quadruplex structure did not negatively affect the recognition of nucleolin by the aptamer. The AS1411-C-8 repressed c-MYC expression at the transcriptional level, possibly due to C-8 ability to stabilize the c-MYC promoter G-quadruplexes. Overall, this study demonstrates the usefulness of AS1411 as a supramolecular carrier of the G-quadruplex binder C-8 and the potential of using its tumour-selective properties for the delivery of ligands for cancer therapy.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10610 - Biophysics
Návaznosti výsledku
Projekt
<a href="/cs/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Strukturní gymnastika nukleových kyselin: od molekulárních principů přes biologické funkce k terapeutickým cílům.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Scientific Reports
ISSN
2045-2322
e-ISSN
—
Svazek periodika
9
Číslo periodika v rámci svazku
MAY 28 2019
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
12
Strana od-do
7945
Kód UT WoS článku
000469217400010
EID výsledku v databázi Scopus
—