Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518314" target="_blank" >RIV/68081707:_____/19:00518314 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1572665719306320?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1572665719306320?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113364" target="_blank" >10.1016/j.jelechem.2019.113364</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces

Popis výsledku v původním jazyce

Two-dimensional (2D) condensation of nucleic acid components at mercury electrodes around the potential of zero charge has been known for decades. In our previous pilot study, we reported on 2D condensation of homopyrimidine oligodeoxynucleotides (ODNs) at the hanging mercury drop electrode (HMDE) in negative potential region. In this work we focused in detail on the effect of the homopyrimidine ODN length (from 3- to 50-mers) on the formation and properties of 2D condensed films at the HMDE. To elicit possible orientation of the homopyrimidine ODN molecules in their 2D condensed layers (i.e. surface concentration of the ODN molecules and the cross-section areas per adsorbed ODN molecules) we used a model in which the maximum surface concentration of the adsorbed ODN molecules is determined from linear dependences of the square of capacitance pit width on temperature. Effect of the length of homoadenine ODNs on their adsorption/desorption processes were studied for comparison, as well as effects of ionic strength and base protonation. Further we focused on the effects of the length of periodically repeated homothymine/homocytosine stretches in 30-mer ODNs on their interfacial behavior at the negatively charged HMDE. Experiments with binary mixtures of noncomplementary and complementary 15-mer homo-ODNs revealed dominance of the homopyrimidine, particularly cytosine, ODN responses in such competitive arrangement. The usefulness of solid silver amalgam electrode for this type of studies is demonstrated. Finally, potential applications of our results in label-free electrochemical analysis of alternative DNA structures are briefly discussed. (C) 2019 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces

Popis výsledku anglicky

Two-dimensional (2D) condensation of nucleic acid components at mercury electrodes around the potential of zero charge has been known for decades. In our previous pilot study, we reported on 2D condensation of homopyrimidine oligodeoxynucleotides (ODNs) at the hanging mercury drop electrode (HMDE) in negative potential region. In this work we focused in detail on the effect of the homopyrimidine ODN length (from 3- to 50-mers) on the formation and properties of 2D condensed films at the HMDE. To elicit possible orientation of the homopyrimidine ODN molecules in their 2D condensed layers (i.e. surface concentration of the ODN molecules and the cross-section areas per adsorbed ODN molecules) we used a model in which the maximum surface concentration of the adsorbed ODN molecules is determined from linear dependences of the square of capacitance pit width on temperature. Effect of the length of homoadenine ODNs on their adsorption/desorption processes were studied for comparison, as well as effects of ionic strength and base protonation. Further we focused on the effects of the length of periodically repeated homothymine/homocytosine stretches in 30-mer ODNs on their interfacial behavior at the negatively charged HMDE. Experiments with binary mixtures of noncomplementary and complementary 15-mer homo-ODNs revealed dominance of the homopyrimidine, particularly cytosine, ODN responses in such competitive arrangement. The usefulness of solid silver amalgam electrode for this type of studies is demonstrated. Finally, potential applications of our results in label-free electrochemical analysis of alternative DNA structures are briefly discussed. (C) 2019 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

849

Číslo periodika v rámci svazku

SEP 15 2019

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

113364

Kód UT WoS článku

000495482900018

EID výsledku v databázi Scopus

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