Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00568949" target="_blank" >RIV/68081707:_____/23:00568949 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00130873

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0013468623000658?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0013468623000658?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2023.141878" target="_blank" >10.1016/j.electacta.2023.141878</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

Popis výsledku v původním jazyce

Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.

Název v anglickém jazyce

Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

Popis výsledku anglicky

Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

1873-3859

Svazek periodika

442

Číslo periodika v rámci svazku

FEB 20 2023

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

141878

Kód UT WoS článku

000923609000001

EID výsledku v databázi Scopus

2-s2.0-85146151102