Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00546616" target="_blank" >RIV/68081707:_____/21:00546616 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00122652

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0013468621014419?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468621014419?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2021.139151" target="_blank" >10.1016/j.electacta.2021.139151</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface

Popis výsledku v původním jazyce

With the increasing interest in DNA G-quadruplexes (G4) as important regulatory elements and prospective therapeutical targets, DNA structure-sensitive electrochemical methods can be advantageously used for studying of their structure or screening for interactions with G4 ligands developed as candidate drugs. Porphyrin derivatives are among the most prominent classes of G4 ligands. Interactions of porphyrins H 2TMPy2PP and Cu-TMPy2PP with oligodeoxynucleotides (ODNs) either forming or not forming G4 were investigated in this work. For this purpose, cyclic voltammetry at hanging mercury drop electrode (HMDE) complemented by UV-vis and CD spectroscopy was applied. Both porphyrins were found to interact strongly with G4 in solution by UV-vis titrations. However, Cu-TMPy2PP appears to interact also with double-stranded and single-stranded ODNs. Accordingly, cyclic voltammetry was able to distinguish between free and ODN-bound forms of Cu-TMPy2PP, but not between binding of the ligand to ODNs with or without G4 propensity. (C) 2021 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface

Popis výsledku anglicky

With the increasing interest in DNA G-quadruplexes (G4) as important regulatory elements and prospective therapeutical targets, DNA structure-sensitive electrochemical methods can be advantageously used for studying of their structure or screening for interactions with G4 ligands developed as candidate drugs. Porphyrin derivatives are among the most prominent classes of G4 ligands. Interactions of porphyrins H 2TMPy2PP and Cu-TMPy2PP with oligodeoxynucleotides (ODNs) either forming or not forming G4 were investigated in this work. For this purpose, cyclic voltammetry at hanging mercury drop electrode (HMDE) complemented by UV-vis and CD spectroscopy was applied. Both porphyrins were found to interact strongly with G4 in solution by UV-vis titrations. However, Cu-TMPy2PP appears to interact also with double-stranded and single-stranded ODNs. Accordingly, cyclic voltammetry was able to distinguish between free and ODN-bound forms of Cu-TMPy2PP, but not between binding of the ligand to ODNs with or without G4 propensity. (C) 2021 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Strukturní gymnastika nukleových kyselin: od molekulárních principů přes biologické funkce k terapeutickým cílům.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

1873-3859

Svazek periodika

394

Číslo periodika v rámci svazku

OCT 20 2021

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

139151

Kód UT WoS článku

000703171900001

EID výsledku v databázi Scopus

2-s2.0-85114124634