Electrochemical detection of G-quadruplex by interaction with porphyrin ligand

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical detection of G-quadruplex by interaction with porphyrin ligand

Popis výsledku v původním jazyce

Porphyrins are well-known binders of DNA and can also specifically target guanine quadruplexes (G4). Interaction of a selected porphyrin (Cu-TMPyPP) with oligonucleotide sequences either forming or not forming G4 was studied using cyclic voltammetry at hanging mercury drop electrode. The interaction is shown to be detectable by cyclic voltammetry as potential shift of a characteristic peak of Cu-TMPyPP which is observed for G4, single stranded as well as double stranded DNA. Nevertheless, further changes of this peak resulting from application of cyclic potential scans to the studied DNA before interaction with Cu-TMPyPP can be used to clearly distinguish G4 DNA, which can be applied as analytical tool for its detection.

Název v anglickém jazyce

Electrochemical detection of G-quadruplex by interaction with porphyrin ligand

Popis výsledku anglicky

Porphyrins are well-known binders of DNA and can also specifically target guanine quadruplexes (G4). Interaction of a selected porphyrin (Cu-TMPyPP) with oligonucleotide sequences either forming or not forming G4 was studied using cyclic voltammetry at hanging mercury drop electrode. The interaction is shown to be detectable by cyclic voltammetry as potential shift of a characteristic peak of Cu-TMPyPP which is observed for G4, single stranded as well as double stranded DNA. Nevertheless, further changes of this peak resulting from application of cyclic potential scans to the studied DNA before interaction with Cu-TMPyPP can be used to clearly distinguish G4 DNA, which can be applied as analytical tool for its detection.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY

ISBN

9788074440687

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

215-219

Název nakladatele

CHARLES UNIV, FAC SCIENCE

Místo vydání

PRAGUE

Místo konání akce

Prague, CZECH REPUBLIC

Datum konání akce

19. 9. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000576787700035