Clustered DNA Damage Formation in Human Cells after Exposure to Low- and Intermediate-Energy Accelerated Heavy Ions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00559818" target="_blank" >RIV/68081707:_____/22:00559818 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1134/S1547477122040227" target="_blank" >https://link.springer.com/article/10.1134/S1547477122040227</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1547477122040227" target="_blank" >10.1134/S1547477122040227</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clustered DNA Damage Formation in Human Cells after Exposure to Low- and Intermediate-Energy Accelerated Heavy Ions

Popis výsledku v původním jazyce

Based on the differentiated analysis of individual radiation-induced foci in three-dimensional images reconstructing the entire volume of the cell nucleus, a detailed analysis of the structure of complex DNA damage clusters in accelerated ion tracks is carried out, and the differences in damage cluster morphology are investigated. It has been found that after exposure to accelerated heavy ions of low and intermediate energies, unlike gamma-rays, complex clusters are formed, including up to six and more individual foci. The obtained results showed that the structure of clustered DNA damage depend on particle LET. The kinetics of the elimination of radiation-induced foci in cells after heavy ion exposure is shown to be slower than after gamma-irradiation. It is concluded that the delay in kinetics is associated with the nature of the microdistribution of heavy charged particles' energy in genetic structures and the formation of complex DNA double-strand breaks (DSBs), which are difficult to repair. A study of the kinetics of radiation-induced foci formation and elimination after exposure to accelerated boron, nitrogen, and neon ions with different physical characteristics has shown that with a decrease in the particles' energy and an increase in their LET, the effectiveness of DNA DSB repair decreases. It is shown that the structure of radiation-induced foci caused by accelerated ions with different physical characteristics is also different. Neon ions with a high density of delta-electrons in the track induce larger and more complex foci clusters.

Název v anglickém jazyce

Clustered DNA Damage Formation in Human Cells after Exposure to Low- and Intermediate-Energy Accelerated Heavy Ions

Popis výsledku anglicky

Based on the differentiated analysis of individual radiation-induced foci in three-dimensional images reconstructing the entire volume of the cell nucleus, a detailed analysis of the structure of complex DNA damage clusters in accelerated ion tracks is carried out, and the differences in damage cluster morphology are investigated. It has been found that after exposure to accelerated heavy ions of low and intermediate energies, unlike gamma-rays, complex clusters are formed, including up to six and more individual foci. The obtained results showed that the structure of clustered DNA damage depend on particle LET. The kinetics of the elimination of radiation-induced foci in cells after heavy ion exposure is shown to be slower than after gamma-irradiation. It is concluded that the delay in kinetics is associated with the nature of the microdistribution of heavy charged particles' energy in genetic structures and the formation of complex DNA double-strand breaks (DSBs), which are difficult to repair. A study of the kinetics of radiation-induced foci formation and elimination after exposure to accelerated boron, nitrogen, and neon ions with different physical characteristics has shown that with a decrease in the particles' energy and an increase in their LET, the effectiveness of DNA DSB repair decreases. It is shown that the structure of radiation-induced foci caused by accelerated ions with different physical characteristics is also different. Neon ions with a high density of delta-electrons in the track induce larger and more complex foci clusters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physics of Particles and Nuclei Letters

ISSN

1547-4771

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

11

Strana od-do

440-450

Kód UT WoS článku

000830723400021

EID výsledku v databázi Scopus

2-s2.0-85134730442