Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00583536" target="_blank" >RIV/68081707:_____/23:00583536 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.degruyter.com/document/doi/10.1515/hsz-2023-0150/html" target="_blank" >https://www.degruyter.com/document/doi/10.1515/hsz-2023-0150/html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/hsz-2023-0150" target="_blank" >10.1515/hsz-2023-0150</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation
Popis výsledku v původním jazyce
Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO(2)OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO(2)OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50?% survival fraction (DERIC50) was calculated for samples pre-treated with NO(2)OA followed by gamma irradiation. NO(2)OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO(2)OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO(2)OA prior irradiation showed a significantly increased DERIC50, demonstrating radiosensitizing effects. In conclusion, NO(2)OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.
Název v anglickém jazyce
Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation
Popis výsledku anglicky
Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO(2)OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO(2)OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50?% survival fraction (DERIC50) was calculated for samples pre-treated with NO(2)OA followed by gamma irradiation. NO(2)OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO(2)OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO(2)OA prior irradiation showed a significantly increased DERIC50, demonstrating radiosensitizing effects. In conclusion, NO(2)OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biological Chemistry
ISSN
1431-6730
e-ISSN
1437-4315
Svazek periodika
2023
Číslo periodika v rámci svazku
SEP 15 2023
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
11
Strana od-do
—
Kód UT WoS článku
001067412100001
EID výsledku v databázi Scopus
2-s2.0-85171733621