Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00618564" target="_blank" >RIV/68081707:_____/24:00618564 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2024.145498" target="_blank" >10.1016/j.electacta.2024.145498</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation
Popis výsledku v původním jazyce
Thymine-mercury-thymine base pair is a well described type of metal ion-mediated interaction in DNA, with a frequent use in construction of biosensors for mercury detection. In this work we took advantage of this interaction to study behavior of thymine-containing oligodeoxynucleotide (ODN) layers adsorbed on the surface of hanging mercury drop electrode (HMDE), which served as a source of mercury cations upon applying a mildly positive potential (+0.15 V). We confirmed formation of the thymine-mercury complex at the surface of HMDE by observing a pair of redox peaks specific for mercury in dependence on presence of the thymine stretches in ODNs. Deoxythymidine mono- or triphosphate gave much weaker signals due to the mercury-thymine complexes. We confirmed that the complex formation can cause unwinding of the AT duplex and concluded that this approach can be used to observe desorption of the homothymine ODN from the HMDE surface. Moreover, we were able to detect stretches of as little as three thymines in ODNs derived from different telomeric repeat sequences.
Název v anglickém jazyce
Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation
Popis výsledku anglicky
Thymine-mercury-thymine base pair is a well described type of metal ion-mediated interaction in DNA, with a frequent use in construction of biosensors for mercury detection. In this work we took advantage of this interaction to study behavior of thymine-containing oligodeoxynucleotide (ODN) layers adsorbed on the surface of hanging mercury drop electrode (HMDE), which served as a source of mercury cations upon applying a mildly positive potential (+0.15 V). We confirmed formation of the thymine-mercury complex at the surface of HMDE by observing a pair of redox peaks specific for mercury in dependence on presence of the thymine stretches in ODNs. Deoxythymidine mono- or triphosphate gave much weaker signals due to the mercury-thymine complexes. We confirmed that the complex formation can cause unwinding of the AT duplex and concluded that this approach can be used to observe desorption of the homothymine ODN from the HMDE surface. Moreover, we were able to detect stretches of as little as three thymines in ODNs derived from different telomeric repeat sequences.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrochimica acta
ISSN
0013-4686
e-ISSN
1873-3859
Svazek periodika
512
Číslo periodika v rámci svazku
FEB 1 2025
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
145498
Kód UT WoS článku
001391577300001
EID výsledku v databázi Scopus
2-s2.0-85212317335