Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00460139" target="_blank" >RIV/68081715:_____/16:00460139 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1556/0806.44.2016.019" target="_blank" >http://dx.doi.org/10.1556/0806.44.2016.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1556/0806.44.2016.019" target="_blank" >10.1556/0806.44.2016.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

Popis výsledku v původním jazyce

Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential.

Název v anglickém jazyce

Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

Popis výsledku anglicky

Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GPP503%2F12%2FP395" target="_blank" >GPP503/12/P395: Hledání glykosylovaných alergenů u potravinářsky významných rostlin pomocí moderních proteomických nástrojů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cereal Research Communications

ISSN

0133-3720

e-ISSN

—

Svazek periodika

44

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

12

Strana od-do

286-297

Kód UT WoS článku

000388000600010

EID výsledku v databázi Scopus

2-s2.0-84971406539