Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00464040" target="_blank" >RIV/68081715:_____/16:00464040 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00159816:_____/16:00066209 RIV/00216224:14110/16:00088929 RIV/26722861:_____/16:N0000026
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s12161-016-0522-6" target="_blank" >http://dx.doi.org/10.1007/s12161-016-0522-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12161-016-0522-6" target="_blank" >10.1007/s12161-016-0522-6</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk
Popis výsledku v původním jazyce
The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.
Název v anglickém jazyce
Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk
Popis výsledku anglicky
The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Food Analytical Methods
ISSN
1936-9751
e-ISSN
—
Svazek periodika
9
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
3251-3257
Kód UT WoS článku
000386668900001
EID výsledku v databázi Scopus
2-s2.0-84966332574