Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00464713" target="_blank" >RIV/68081715:_____/16:00464713 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/16:00089213

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2015.12.009" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.12.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2015.12.009" target="_blank" >10.1016/j.electacta.2015.12.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Popis výsledku v původním jazyce

Gelatin-templated gold nanostructures were fabricated in order to improve the sensitivity of nonenzymatic determination of glucose. The three-dimensional cavities were created by crosslinking of gelatin layer on the electrode and the specific saw tooth-like morphology of surface was formed by electrodeposition of gold through the cavities. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the investigation of nanostructure growth and characterization of prepared surfaces. Glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alkaline solution with excellent sensitivity of 1165 mA mM 1cm 2, wide linear range from 32 mM to 10 mM and limit of detection (LOD) of 90 mM. During amperometric measurements LOD of 1.5 mM was achieved. The sensor was able to detect real concentrations of glucose in deproteinized human serum with negligible interference of oxidizable blood components. Great correlation with results obtained from reference methods (commercial glucometer and standard kit for photometric glucose detection) was demonstrated; the determination of glucose in serum was performed with relative standard deviation of 4%. The sensor exhibits a strong potential as a rapid and sensitive non-enzymatic approach in future applications for clinical analyses.

Název v anglickém jazyce

Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Popis výsledku anglicky

Gelatin-templated gold nanostructures were fabricated in order to improve the sensitivity of nonenzymatic determination of glucose. The three-dimensional cavities were created by crosslinking of gelatin layer on the electrode and the specific saw tooth-like morphology of surface was formed by electrodeposition of gold through the cavities. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the investigation of nanostructure growth and characterization of prepared surfaces. Glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alkaline solution with excellent sensitivity of 1165 mA mM 1cm 2, wide linear range from 32 mM to 10 mM and limit of detection (LOD) of 90 mM. During amperometric measurements LOD of 1.5 mM was achieved. The sensor was able to detect real concentrations of glucose in deproteinized human serum with negligible interference of oxidizable blood components. Great correlation with results obtained from reference methods (commercial glucometer and standard kit for photometric glucose detection) was demonstrated; the determination of glucose in serum was performed with relative standard deviation of 4%. The sensor exhibits a strong potential as a rapid and sensitive non-enzymatic approach in future applications for clinical analyses.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

—

Svazek periodika

188

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

277-285

Kód UT WoS článku

000370986500032

EID výsledku v databázi Scopus

2-s2.0-84949604623