Theory of electrophoretic focusing on aninverse electromigration dispersion profile

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00524029" target="_blank" >RIV/68081715:_____/20:00524029 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0308343" target="_blank" >http://hdl.handle.net/11104/0308343</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900229" target="_blank" >10.1002/elps.201900229</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Theory of electrophoretic focusing on aninverse electromigration dispersion profile

Popis výsledku v původním jazyce

Electrophoretic focusing on an inverse electromigration dispersion (EMD) profile is basedon a principle different from those of other electrophoretic separation methods. It hasalready proved its applicability in analytical practice by offering competitive separationperformance and sensitivity and specific selectivity. It can be classified as an intermedi-ate between field-driven and equilibrium gradient methods and is therefore interestingfrom the viewpoint of theory of separation methods. This work presents a comprehensivetheoretical description of electrophoretic focusing on an inverse EMD profile compris-ing properties of the electrolyte system, formed gradients, and focused analyte zones. Theseparation properties are described in terms of resolution and peak capacity and their de-pendence on system and analyte properties is discussed from the viewpoint of how thecounteracting phenomena of electromigration and dispersion are affected by electric cur-rent, voltage, and hydrodynamic and electroosmotic flow. The overall performance of thepresent method is shown to be comparable with other electrophoretic separation methodslike zone electrophoresis or isoelectric focusing.

Název v anglickém jazyce

Theory of electrophoretic focusing on aninverse electromigration dispersion profile

Popis výsledku anglicky

Electrophoretic focusing on an inverse electromigration dispersion (EMD) profile is basedon a principle different from those of other electrophoretic separation methods. It hasalready proved its applicability in analytical practice by offering competitive separationperformance and sensitivity and specific selectivity. It can be classified as an intermedi-ate between field-driven and equilibrium gradient methods and is therefore interestingfrom the viewpoint of theory of separation methods. This work presents a comprehensivetheoretical description of electrophoretic focusing on an inverse EMD profile compris-ing properties of the electrolyte system, formed gradients, and focused analyte zones. Theseparation properties are described in terms of resolution and peak capacity and their de-pendence on system and analyte properties is discussed from the viewpoint of how thecounteracting phenomena of electromigration and dispersion are affected by electric cur-rent, voltage, and hydrodynamic and electroosmotic flow. The overall performance of thepresent method is shown to be comparable with other electrophoretic separation methodslike zone electrophoresis or isoelectric focusing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-13135S" target="_blank" >GA18-13135S: Využití kapilární elektroforézy při analýze suchých krevních skvrn</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

471-480

Kód UT WoS článku

000490359900001

EID výsledku v databázi Scopus

2-s2.0-85073931643