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Preparation and Analysis of Quantum Dots: Applications of Capillary Electrophoresis

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00544799" target="_blank" >RIV/68081715:_____/20:00544799 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/00216305:26620/20:PU136515

  • Výsledek na webu

    <a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-0463-2_3#citeas" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-0463-2_3#citeas</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-0716-0463-2_3" target="_blank" >10.1007/978-1-0716-0463-2_3</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Preparation and Analysis of Quantum Dots: Applications of Capillary Electrophoresis

  • Popis výsledku v původním jazyce

    The implementation of quantum dots in analytical chemistry has already advanced from basic research activities to routine applications of commercially available fluorescent agents present in sophisticated assays kits. Nevertheless, a further development of new preparation and characterization methods of nanoparticles is still required to increase the sensitivity of analytical methods substantially. Thus, in many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach even the absolute sensitivity, that is, the capability to detect a single molecule in complex matrices. Semiconductor quantum dots have already proved to be suitable components of highly luminescent tags, probes, and sensors with broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with wide ranges of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and low photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed CdTe quantum dots, their coatings and conjugation reactions with antibodies, DNA, and macrocycles, and their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies and immunocomplexes with targeted antigens, is demonstrated as an example.

  • Název v anglickém jazyce

    Preparation and Analysis of Quantum Dots: Applications of Capillary Electrophoresis

  • Popis výsledku anglicky

    The implementation of quantum dots in analytical chemistry has already advanced from basic research activities to routine applications of commercially available fluorescent agents present in sophisticated assays kits. Nevertheless, a further development of new preparation and characterization methods of nanoparticles is still required to increase the sensitivity of analytical methods substantially. Thus, in many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach even the absolute sensitivity, that is, the capability to detect a single molecule in complex matrices. Semiconductor quantum dots have already proved to be suitable components of highly luminescent tags, probes, and sensors with broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with wide ranges of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and low photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed CdTe quantum dots, their coatings and conjugation reactions with antibodies, DNA, and macrocycles, and their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies and immunocomplexes with targeted antigens, is demonstrated as an example.

Klasifikace

  • Druh

    C - Kapitola v odborné knize

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název knihy nebo sborníku

    Quantum Dots

  • ISBN

    978-1-0716-0462-5

  • Počet stran výsledku

    29

  • Strana od-do

    "Roč. 2135 (2020)"

  • Počet stran knihy

    308

  • Název nakladatele

    Humana Press

  • Místo vydání

    New York

  • Kód UT WoS kapitoly

    000680965100004