Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F22%3A00565880" target="_blank" >RIV/68081715:_____/22:00565880 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/22:00127321
Výsledek na webu
<a href="https://hdl.handle.net/11104/0337362" target="_blank" >https://hdl.handle.net/11104/0337362</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.2c03543" target="_blank" >10.1021/acs.analchem.2c03543</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Popis výsledku v původním jazyce
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
Název v anglickém jazyce
Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Popis výsledku anglicky
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Svazek periodika
94
Číslo periodika v rámci svazku
47
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
16376-16383
Kód UT WoS článku
000889895000001
EID výsledku v databázi Scopus
2-s2.0-85142460861