Micro-electromembrane extraction through volatile free liquid membrane for the determination of β-lactam antibiotics in biological and environmental samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00560227" target="_blank" >RIV/68081715:_____/23:00560227 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0333236" target="_blank" >https://hdl.handle.net/11104/0333236</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2022.123831" target="_blank" >10.1016/j.talanta.2022.123831</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micro-electromembrane extraction through volatile free liquid membrane for the determination of β-lactam antibiotics in biological and environmental samples

Popis výsledku v původním jazyce

Micro-electromembrane extraction (μ-EME) was presented for the selective extraction of four main β-lactam antibiotics (penicillin, phenoxypenicillin, ampicillin, and amoxicillin) from complex samples. A volatile solvent (ethyl acetate or chloroform) was sandwiched between a plug of the complex sample and another plug of an aqueous acceptor solution in a transparent polymeric tube and formed the so-called free liquid membrane (FLM). The use of the FLM eliminated the evaporation of the solvent and enabled the μ-EME of the antibiotics, which was carried out by the application of DC voltage to the terminal aqueous solutions. The drugs in the complex sample were selectively transferred through the FLM to the acceptor solution, which was directly used for their determination by micellar electrokinetic chromatography with ultraviolet detection (MEKC-UV). The μ-EME was characterized by sub-μA electric currents, high elimination of matrix components, high stability of operational solutions, and suitability for extracting undiluted complex samples. The μ-EME/MEKC-UV method yielded good analytical repeatability (RSDs of peak areas ≤5%), extraction recoveries (40–84%), accuracy (92–105%) and linearity over one and a half order of magnitude (R2 ≥ 0.9998), and was applied to the determination of the four β-lactam antibiotics in human serum and waste water at clinically and environmentally relevant concentration levels. Further improvement in the method sensitivity was achieved by changing the μ-EME tube geometryn(conical shape) and increasing the complex sample volume (100 μL). The analytes were enriched by factors of 7.6–11.5, the limits of detection dropped down to less than 18 ng/mL, and the modified μ-EME/MEKC-UV method enabled the trace determination of β-lactam antibiotics in complex samples.

Název v anglickém jazyce

Micro-electromembrane extraction through volatile free liquid membrane for the determination of β-lactam antibiotics in biological and environmental samples

Popis výsledku anglicky

Micro-electromembrane extraction (μ-EME) was presented for the selective extraction of four main β-lactam antibiotics (penicillin, phenoxypenicillin, ampicillin, and amoxicillin) from complex samples. A volatile solvent (ethyl acetate or chloroform) was sandwiched between a plug of the complex sample and another plug of an aqueous acceptor solution in a transparent polymeric tube and formed the so-called free liquid membrane (FLM). The use of the FLM eliminated the evaporation of the solvent and enabled the μ-EME of the antibiotics, which was carried out by the application of DC voltage to the terminal aqueous solutions. The drugs in the complex sample were selectively transferred through the FLM to the acceptor solution, which was directly used for their determination by micellar electrokinetic chromatography with ultraviolet detection (MEKC-UV). The μ-EME was characterized by sub-μA electric currents, high elimination of matrix components, high stability of operational solutions, and suitability for extracting undiluted complex samples. The μ-EME/MEKC-UV method yielded good analytical repeatability (RSDs of peak areas ≤5%), extraction recoveries (40–84%), accuracy (92–105%) and linearity over one and a half order of magnitude (R2 ≥ 0.9998), and was applied to the determination of the four β-lactam antibiotics in human serum and waste water at clinically and environmentally relevant concentration levels. Further improvement in the method sensitivity was achieved by changing the μ-EME tube geometryn(conical shape) and increasing the complex sample volume (100 μL). The analytes were enriched by factors of 7.6–11.5, the limits of detection dropped down to less than 18 ng/mL, and the modified μ-EME/MEKC-UV method enabled the trace determination of β-lactam antibiotics in complex samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

252

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

123831

Kód UT WoS článku

000860344000007

EID výsledku v databázi Scopus

2-s2.0-85136123626