Preparativeseparationof immunoglobulins from bovine colostrum by continuous divergent-flow electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00562904" target="_blank" >RIV/68081715:_____/23:00562904 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0335073" target="_blank" >https://hdl.handle.net/11104/0335073</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202200679" target="_blank" >10.1002/jssc.202200679</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparativeseparationof immunoglobulins from bovine colostrum by continuous divergent-flow electrophoresis

Popis výsledku v původním jazyce

Immunoglobulins in bovine colostrum were separated and fractionated from other proteins using method and instrumentation developed in our laboratory. The proposed separation was based on bidirectional isotachophoresis/moving boundary electrophoresis with electrofocusing of the analytes in pH gradient from 3.9 to 10.1. The preparative instrumentation included the trapezoidal non-woven fabric that served as separation space with divergent continuous flow. The defatted and casein precipitate free colostrum supernatant was loaded directly into instrument without any additional colostrum pre-preparation. Immunoglobulin G that was fractionated from other immune proteins such as bovine serum albumin, β-lactoglobulin and α-lactalbumin, was continuously collected in separated fractions over 3 hours. The fractions were further processed, and isolated immunoglobulin G in the liquid fractions was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by re-focusing in gel isoelectric focusing. Separated immunoglobulin G was detected in seven fractions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with gradually decreased concentration in the fractions. Re-focusing of the proteins in the fractions by gel isoelectric focusing revealed multiple separated zones of immunoglobulin G with the pI values covering the range from 5.4 to 7.2. Each fraction contained the distinct zones with gradually increased pI values and decreased concentrations from fraction to fraction.

Název v anglickém jazyce

Preparativeseparationof immunoglobulins from bovine colostrum by continuous divergent-flow electrophoresis

Popis výsledku anglicky

Immunoglobulins in bovine colostrum were separated and fractionated from other proteins using method and instrumentation developed in our laboratory. The proposed separation was based on bidirectional isotachophoresis/moving boundary electrophoresis with electrofocusing of the analytes in pH gradient from 3.9 to 10.1. The preparative instrumentation included the trapezoidal non-woven fabric that served as separation space with divergent continuous flow. The defatted and casein precipitate free colostrum supernatant was loaded directly into instrument without any additional colostrum pre-preparation. Immunoglobulin G that was fractionated from other immune proteins such as bovine serum albumin, β-lactoglobulin and α-lactalbumin, was continuously collected in separated fractions over 3 hours. The fractions were further processed, and isolated immunoglobulin G in the liquid fractions was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by re-focusing in gel isoelectric focusing. Separated immunoglobulin G was detected in seven fractions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with gradually decreased concentration in the fractions. Re-focusing of the proteins in the fractions by gel isoelectric focusing revealed multiple separated zones of immunoglobulin G with the pI values covering the range from 5.4 to 7.2. Each fraction contained the distinct zones with gradually increased pI values and decreased concentrations from fraction to fraction.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

1615-9314

Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

2200679

Kód UT WoS článku

000876663100001

EID výsledku v databázi Scopus

2-s2.0-85141364876