Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00579986" target="_blank" >RIV/68081715:_____/23:00579986 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/86652036:_____/23:00579986 RIV/61388971:_____/23:00579986
Výsledek na webu
<a href="https://www.nature.com/articles/s41598-023-41567-7" target="_blank" >https://www.nature.com/articles/s41598-023-41567-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-023-41567-7" target="_blank" >10.1038/s41598-023-41567-7</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
Popis výsledku v původním jazyce
In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA.
Název v anglickém jazyce
Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
Popis výsledku anglicky
In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Svazek periodika
13
Číslo periodika v rámci svazku
September 6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
14
Strana od-do
14669
Kód UT WoS článku
001138051400001
EID výsledku v databázi Scopus
2-s2.0-85170103294