Adapting the laser-induced fluorescence detection setup of the standard capillary electrophoresis equipment to achieve high-sensitivity detection of 2-aminoacridone labeled oligosaccharides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00617403" target="_blank" >RIV/68081715:_____/25:00617403 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00141057

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.70112" target="_blank" >10.1002/jssc.70112</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adapting the laser-induced fluorescence detection setup of the standard capillary electrophoresis equipment to achieve high-sensitivity detection of 2-aminoacridone labeled oligosaccharides

Popis výsledku v původním jazyce

The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.

Název v anglickém jazyce

Adapting the laser-induced fluorescence detection setup of the standard capillary electrophoresis equipment to achieve high-sensitivity detection of 2-aminoacridone labeled oligosaccharides

Popis výsledku anglicky

The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

1615-9314

Svazek periodika

48

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

e70112

Kód UT WoS článku

001445198900001

EID výsledku v databázi Scopus

2-s2.0-105000445466