Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00587086" target="_blank" >RIV/68081715:_____/24:00587086 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf" target="_blank" >https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF

Popis výsledku v původním jazyce

Fluorescent labeling represents a key step in oligosaccharide and glycan analysis by capillary electrophoresis with laser-induced fluorescence detection. In this work, we present a synthesis of the rhodamine B-based fluorescent tag modified by a hydrazide functionality for saccharide derivatization via hydrazone formation chemistry. The labeling conditions were optimized to obtain a high reaction yield. The labeled oligosaccharides and glycans were separated in polybrene-coated capillaries and the separation conditions (BGE compositions, separation, voltage, capillary length, etc.) were optimized. The labeled products were detected with a 532-nm laser and 550-nm longpass or 560/10-nm bandpass optical filters. The optimized labeling and separation conditions were applied for CE/LIF analysis of glycans released from model glycoproteins such as ribonuclease B and immunoglobulin G.

Název v anglickém jazyce

Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF

Popis výsledku anglicky

Fluorescent labeling represents a key step in oligosaccharide and glycan analysis by capillary electrophoresis with laser-induced fluorescence detection. In this work, we present a synthesis of the rhodamine B-based fluorescent tag modified by a hydrazide functionality for saccharide derivatization via hydrazone formation chemistry. The labeling conditions were optimized to obtain a high reaction yield. The labeled oligosaccharides and glycans were separated in polybrene-coated capillaries and the separation conditions (BGE compositions, separation, voltage, capillary length, etc.) were optimized. The labeled products were detected with a 532-nm laser and 550-nm longpass or 560/10-nm bandpass optical filters. The optimized labeling and separation conditions were applied for CE/LIF analysis of glycans released from model glycoproteins such as ribonuclease B and immunoglobulin G.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/9F23002" target="_blank" >9F23002: Development of High Sensitivity and High Resolution Analytical Tools for the Brain Glycans</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů