Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00603181" target="_blank" >RIV/68081715:_____/25:00603181 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00140682

Výsledek na webu

<a href="https://hdl.handle.net/11104/0360419" target="_blank" >https://hdl.handle.net/11104/0360419</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2024.127456" target="_blank" >10.1016/j.talanta.2024.127456</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection

Popis výsledku v původním jazyce

In this work, we present the synthesis and application of fluorescent rhodamine B hydrazide for the derivatization of simple oligosaccharides and complex glycans using a hydrazone formation chemistry approach. The labeling conditions and the experimental setup of CE/LIF were optimized by analyzing oligosaccharide standards. The CE/LIF separations were performed in polybrene-coated capillaries eliminating the need for the purification step after derivatization. The addition of methanol to the background electrolyte significantly increased the LIF detection sensitivity reaching the limits of detection in the attomole range. The resolution of carbohydrate samples was improved by using long (98 cm) capillaries and polymer additives (polybrene). The developed method was applied for CE/LIF and CE-MS analysis of N-linked glycans released from bovine ribonuclease B and the therapeutic monoclonal antibody of trastuzumab.

Název v anglickém jazyce

Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection

Popis výsledku anglicky

In this work, we present the synthesis and application of fluorescent rhodamine B hydrazide for the derivatization of simple oligosaccharides and complex glycans using a hydrazone formation chemistry approach. The labeling conditions and the experimental setup of CE/LIF were optimized by analyzing oligosaccharide standards. The CE/LIF separations were performed in polybrene-coated capillaries eliminating the need for the purification step after derivatization. The addition of methanol to the background electrolyte significantly increased the LIF detection sensitivity reaching the limits of detection in the attomole range. The resolution of carbohydrate samples was improved by using long (98 cm) capillaries and polymer additives (polybrene). The developed method was applied for CE/LIF and CE-MS analysis of N-linked glycans released from bovine ribonuclease B and the therapeutic monoclonal antibody of trastuzumab.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

286

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

127456

Kód UT WoS článku

001412428700001

EID výsledku v databázi Scopus

2-s2.0-85213545134